Quiz #4 Flashcards
What are the structures in the endomembrane system?
Nuclear envelope Endoplasmic reticulum Vacuoles Vesicles Lysosomes Golgi Plasma membrane
How are the structures in the endomembrane system related?
physical continuity
vesicles that transfer from one to another
What structures are not in the endomembrane system?
peroxisomes, mitochondria, and chloroplasts
What is the nucleus?
stores DNA, and is the site of transcription
contains multiple linear chromosomes
outer membrane is continuous with the endoplasmic reticulum
What is the nuclear envelope?
is a double membrane (each membrane is a phospholipid bilayer)
separates the nucleoplasm from the cytoplasm
What are nuclear pores?
complexes span both bilayers
regulates movement of substances in and out of nucleus
What is the nuclear lamina?
provides structural support for the nucleus
composed of an immediate filament called lamin, which assembles on the inner surface of the nuclear envelope
What is chromatin?
DNA and protein
chromatin can be highly condensed
chromatin becomes even more packaged in mitosis, to prepare for chromatid segregation
Why is DNA packaged?
organize the nucleus
protect the DNA from damage
make it easier to move replicated DNA into daughter cell during cell division
What is histone?
provides stability and protection to DNA, and it is involved in the packaging of nucleosomes into the 30 nm fibre
4 histone proteins (H2A, H2B, H3, H4) assemble into a larger 8-subunit complex called a histone octamer
DNA + histone octamer = nucleosome
What is the nucleolus?
this is a visible sub-compartment of the nucleus (not bound by membrane)
site of the ribosomal RNA (rRNA) synthesis
site of assembly of ribosome protein subunits (each half of the ribosome enters the nucleus separately via nuclear pores)
How is 5S RNA is made in the nucleus?
- Each subunit (made in the cytoplasm) must enter nucleus
- Ribosomal subunits associate with 4 different RNA molecules
- Final assembly occurs in the cytoplasm
What is the endoplasmic reticulum?
2 types: smooth and rough
the two types of ER often appear separate, but they are sub-compartments of the same organelle
both types are in plant and animal cells
cells that produce a lot of proteins will tend to have more rough ER
cells that produce a lot of lipids (fats) and steroid hormones will have more smooth ER
What is rough ER?
covered in ribosomes
rER ribosomes are associated with the translation of proteins that are destined for the endomembrane system
site of protein synthesis and assembly
site of protein quality control
What are ribosomes?
protein factories
particles made of ribosomal RNA and protein
assemble all peptides (using amino acid monomers)
in the cytosol
on the outside of endoplasmic reticulum
What is smooth ER?
ion storage
phospholipid, steroid synthesis
detox of drugs and alcohol
carbohydrate metabolism
What is the Golgi apparatus?
consists of flattened membranous sacs called cisternae
cis face = “receiving” side of the golgi (faces nucleus)
cisternal maturation: golgi cisternae move in a cis to trans direction
Vesicles form and leave the golgi, carrying products to different membranous organelles, or the plasma membrane
trans face = “shipping” side of the golgi (faces away from the nucleus)
What is the function of the golgi apparatus?
modifies products of the ER
changing carbohydrates on proteins, phospholipids
sorts and packages materials into transport vesicles
it manufactures some macromolecules
What are lysosomes?
is a membranous sac of hydrolytic enzymes that can digest macromolecules
lysosomal enzymes work best in acidic environment inside lysosome
lysosomal enzymes can hydrolyze proteins, fats, polysaccharides, and nucleic acids
also important for recycling various structures and organelles
What is autophagy?
“self-eating”
the cell eats itself (or parts of itself)
What are vacuoles?
variable function depending on the cell
food vacuoles: formed by phagocytosis
contractile vacuoles: found in many freshwater protists, pump excess water out of cells
central vacuole: specific organelle of plants, found in many mature plant cells, holds organic compounds and water (helps maintain turgor pressure)
What do mitochondria and chloroplasts have in common?
both bound by a double membrane
both contain several copies of their own DNA
both contain ribosomes that synthesize a few compounds, but most of the components in each organelle are encoded by genes in the nucleus
What are perxisomes?
also called microbodies
single membrane bound organelles (spherical)
perform reactions (breakdown of molecules or assembly) in which H2O2 (hydrogen peroxide) is a by-product
H2O2 is toxic to the cell
fatty acids + O2 –> smaller products + H2O2 –> H2O + 1/2 O2