Quiz 2 Flashcards
FRAP
Fluorescence Recovery After Photobleaching. Molecular cloning, photobleaching an area that removes fluorescence. Rate of protein movement studied by how fast the proteins restore fluorescence.
FRET
Fluorescence Resonance Energy Transfer
2 Different proteins undergo molecular coding with tracers next to each other on color wheel. Fluorescent light excitations will emit a color 2 off on the wheel if proteins are interacting. Determines interactions between each protein.
Electron microscopy
best res, electron beam for energy, fixed cells only, electron detector and screen used. specimen receives metal salts stain and beam collects salts.
SEM
Scanning Electron Microscopy, creates image from detecting reflected or knocked off electrons
TEM
Transmission Electron Microscopy, observes the electrons transmitting through the sample
4 Methods for generating lysate
Sonication - sound is used to break PM
Detergent used to make holes in PM
Pushing cell through small hole at high pressure to tear open PM
Homogenization - grinder or blender breaks PM, mortar and pestle
Pellet in each centrifugation
1st Nuclei
2nd Mitochondria, lysosomes, peroxisomes
3rd PM and ER
4th Ribosomes
Totipotent SC
unlimited capability, fertilized egg
Pluripotent
gives rise to some but not all cells
multipotent
gives rise to cells with a particular function
Hematopoietic SC
Multipotent, found in bone marrow that produce lymphoid and myeloid progenitors
Lymphoid prog. can produce
B, T, NK, lymphoid dendritic
Myeloid prog. can produce
macrophages, eosinophil, neutrophil, basophil, platelets, myeloid dendritic, erythrocytes
Intestinal Epithelial Cells
intestines are lined with wavy sheets of cells, peaks villus, bottoms crypt. bottom has multipotent SCs that make 3 types of cells
3 types of IE cells differentiated
Goblet, Absorptive Epithelial, Enteroendocrine cells
