Quiz 1 midterms Flashcards
basic unit of kidneys
Nephrons
- main filtering
Glomerulus
Shield of negativity
Fenestrated membrane
Parts of the glomerulus that filters
Codocytes, and fenestrated membrane
Major site of reabsorption
Proximal convoluted tubule
where urine concentration
begins/starts
Loop of Henle
ADH acts on;
final urine concentration starts
Distal convoluted tubule
final urine concentration
Collecting duct
produces ADH
Adrenal cortex
produces renin
Juxtaglomerular apparatus
Normal Body Mechanism (BH, ADH, UV, UC)
I body hydration = D ADH = I urine volume = D urine concentration
D body hydration = I ADH = D urine volume = I urine concentration
Diabetes insipidus mechanism (BH, ADH, UV, UC)
D body hydration = D ADH = I urine volume = D urine concentration
Average daily output of urine
1200mL/day
Normal renal plasma flow
600-700mL/minute
types of urine in Routine Urinalysis
-random urine
-first-morning urine
-mid-stream clean catch urine
type of urine for Bacterial Culture
catheterized
-suprapubic - most sterile
-mid-stream clean catch
Bottle/Container characteristic and size
-clear
-wide-mouth
-leak-proof
Capacity:50mL
In Unpreserved Urine, what will be increased?
pH: (unpreserved:9)
-bacteria
-odor
-nitrite
For microscopic what ml of urine?
12 ml
Nitrite will be increase because of?
Bacteria
In Unpreserved Urine, what will be decrease?
-bilirubin
-urobilinogen
-cast
-clarity
-cells
-ketones
-glucose
photooxidation into biliverdin
bilirubin
photooxidation into urobilin
urobilinogen
disintegrate in alkaline urine
cast
bacteria multiplication
clarity
Three parts of routine urinalysis in
Physical
Odor, color, and clarity
odor of phenylketonuria
mousy
odor of isovaleric
acidemia
sweaty feet
odor of diabetes
fruity
No visible particulates, transparent
Clear
Few particulates, newsprint easily seen through urine
Hazy
Many particulates, newsprint is blurred through urine
Cloudy
Newsprint cannot be seen
Turbid
Specific gravity of urine
1.003-1.035
May precipitate or clot
Milky
to protect the chemicals from the sunlight, tightly sealed,
every after use covers it, prevent run over of
analytes of reagent strip
Amber color
Give me the seconds, NV of LE
120 seconds;
NV:0-5/HPF
It is a component of the reagent strip that is 60 seconds and aid in
diagnosis of liver diseases and hemolytic disorders; Give me also the NV
Urobilinogen, NV: <1 mg/dL
It is a component of the reagent strip that is 60 seconds and do not usually appear in the urine; increased nitrite
is related with (?)
Nitrite, UTI
It is a component of the reagent strip that is 60 seconds and causes pre-renal, renal (orthostatic proteinuria), post-renal; Give me also the NV
Protein,NV: <10
mg/dL
clinical proteinuria has value in protein?
> 30 mg/dL
pH morning urinerange and Normal urine range
pH of 5-6; pH of 4.5-8
Blood has what seconds and NV?
60, 0-4/HPF
hematuria color
cloudy urine
myoglobinuria
clear red urine
most common cause of myoglobin
rhabdomyolysis
What is the second of SG and what will be color if increase and decrease?
45 seconds; increased SG
(darker) and decreased SG (lighter)
measurable
amount of ketones do not appear in
urine but it shows deficiency in
insulin; highly correlated with
glucose
Ketones, 40 seconds
conjugated bilirubin; detect post hepatic (bile
duct obstruction) or hepatic (liver
damage) jaundice; How many seconds
Bilirubin, 30 seconds
urine contains only small amount of
glucose; increased in DM; How many seconds
Glucose, 30 sec
LPF
Casts, crystals, epithelial cells, etc
HPF
WBCs, RBCs, bacteria, etc
May indicate the presence of certain sediments
Color
Pathologic or non-pathologic causes of turbidity
Clarity
RBCs, RBC Cast
Blood
Casts, cells
Protein
Bacteria, WBCs
Nitrite
WBCs, WBC Casts, bacteria
LE
Yeast
Glucose
Specimen Preparation and Examination, decant the urin to remains?
(0.5-1 mL remains)
Specimen Preparation and Examination, how much mL of urin needed?
10 to 15 mL
Transfer (?) uL to glass slide with (?)
20ul, 22 x 22 mm coverslip
Quantitative measure of
formed elements using a
hemocytometer
Addis Count
What specimen used in Addis count?
12 hour urine
What preservative used in Addis count?
NaF (Sodium fluoride)
Normal Values of RBC
0 - 500,000
Normal Values of WBCs & ECs
0 - 1,800,000
Normal Values of Hyaline Casts
0 - 5,000
Sternheimer-Malbin is made up of?
(CV + Safranin
O)
Enhances nuclear
detail
0.5% Toluidine
Blue
Delineates structure
and contrasting
colors of the nucleus
and cytoplasm
SternheimerMalbin
Lyses RBCs and
enhances nucleus of
WBCs
2% Acetic acid
Stains TAG and
neutral fats and what will be the color?
Lipid stains, orange red
Lipid stain is composed of?
(ORO and Sudan
III)
Differentiates Gram
(-) to Gram (+)
bacteria
GS
Stains eosinophilic
granules
Hansel Stain
Stains sediments
containing iron
Prussian Blue
Hansel Stain is composed of?
(Eosin Y +
Methylene
blue)
Function of SternheimerMalbin
WBCs, ECs, and casts
Function of 0.5% Toluidine
Blue
Differentiates WBCs
and RTE cells
Function of 2% Acetic acid
Distinguishes RBCs
from WBCs, yeasts, oil
droplets and crystals
Function of Lipid stains
Identifies free fat
droplets and lipidcontaining cells &
casts
Function of GS
Bacterial casts
Function of Hansel Stain
Urinary eosinophils
Function of Prussian Blue
Identifies hemosiderin
granules in casts and
cells
Used for routine urinalysis
Bright-Field Microscopy
Visualization of elements with low
refractive indices (hyaline, mixed cellular
cast, MT, Trichomonas)
Phase-Contrast Microscopy
Identification of cholesterol molecules in
OFB, fatty casts and crystals
Polarizing Microscopy
For fluorescent microorganisms or those
stained with a fluorescent dye
Fluorescence Microscopy
Identification of Treponema spp
Dark-Field Microscopy
3-D microscopy image and layer-by-layer
imaging of a specimen
Interference-Contrast
Microscopy
Microscope for the differential?
Nomarski
Microscope for the Modulation?
Hoffmann
What is per HPF
Crystals (normal)
Bacteria
RBCs
WBCs
OFB
Abnormal crystals and casts
What is per LPF
Epithelial Cell
Mucus Thread
Casts
SEC
Abnormal crystal and cast
What is per 10 HPF
RTE cells
What component if for the color of urine?
Urochrome
Centrifuge at (?) RCF for (?) mins
400 RCF, 5 min
400 RCF is what in RPM?
1200-1500 RPM
Examine how many LPF and HPF
10 LPF, and 10 HPF