Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology 211 Lab > Quiz 1 > Flashcards

Quiz 1 Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

What is biosafety?

A

It is a general protocol used in laboratory to prevent exposure to contaminated culture/bacteria.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is biosafety level 1?

A

In BSL 1, we are working with microbes that present little to no threat to us. We must still make sure to wear protective gear (lab coat and eye protection) and disinfect lab bench and wash hands.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is biosafety level 2?

A

In BSL 2, we will often work with microorganisms associated with human disease. We have to follow BSL 1 safety procedures and also make sure that we were gloves and work in a sterile environment.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is biosafety level 3?

A

In BSL 3, we will work with diseases that may have serious or lethal consequences. We follow BSL 2 and make sure decontamination occurs. We will have to wear lab coat, eye protection, gloves and respiratory protection.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What goes inside the sharps container?

A

Only sharp glass objects

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What goes inside the step on can (biohazard)?

A

Only for soft contaminated materials (gloves, paper, cotton etc)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is the pipette jar for?

A

Only for reusable glass pipettes.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What do we use to clean bright-field microscope?

A

lens paper

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

How do we store the microscope?

A

Make sure to clean lens, turn light off, lower stage, wrap cord, turn to 4x magnification and use both hands to return to the proper number slot

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is brightfield microscope used for? What is one advantage?

A

Since we work with small samples, we make sure to place them in a bright background so they are easier to view. That is why we use them for staining procedures

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is dark field microscope used for? What is one advantage?

A

We use this when working with unstained specimens and it is good because they are light organisms viewed under a dark background.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is the phase contrast microscope used for? What is one advantage?

A

Phase-contrast microscopy is a technique used for gaining contrast in a translucent specimen without staining the specimen. We are able to see internal structures and are mainly for living cells.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is fluorescent microscopy used for? What is one advantage

A

Fluorescent microscopy is often used to image specific features. It is also used to visually enhance 3-D features at small scales.

It has a highly specific fluorescent stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is electron microscopy used for? What is one advantage

A

is a technique for obtaining high resolution images of biological and non-biological specimens. It is used in research to investigate the structure of tissues, cells, organelles, etc.
PRO: much higher magnification and resolution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is cyanobacteria?

A

microscopic organisms found naturally in all types of water. Most of them form colonies and obtain their food like photosynthesis. They are a oxygen producing prokaryote.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is algae?

A

They fall within the eukaryotes category and can be both unicellular and multicellular. They also perform photosynthesis, they do this with chloroplasts

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What is protozoa?

A
  • Single-celled eukaryotes
  • Most lack cell walls
  • Uses organic carbon for growth by consuming other organisms
  • Some are human parasites
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

List 4 differences between eukaryote and prokaryote

A

Eukaryotes are bigger, more complex cells with membrane bound organelles and cytoskeleton.
Prokaryotes are smaller, simpler cells with no membrane bond organelles and no cytoskeleton

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What is a parasite?

A

an organism that lives in another organism (host) and benefits by deriving nutrients at the other’s expense.

20
Q

What is the purpose of aseptic technique?

A

Safe handling and transfer of bacterial cultures

Avoid contamination of bacterial culture by unwanted organisms

21
Q

What are the 3 types of motility for bacteria

A

Flagellates
Ameboid
Ciliates

Ex: flagella
Amoba,

22
Q

What are the 3 types of motility for bacteria

A

Flagellates
Ameboid
Ciliates

Ex: flagella
Amoba,

23
Q

What is the purpose of bunsen burner?

A

To make sure all of our tools are sterilized before using them

24
Q

What is the purpose of inoculating loop?

A

To pick up broth species

25
Q

What is the purpose of inoculating needle?

A

To pick up agar species

26
Q

What is culture medium?

A

Nutrient material for the growth of microbes. (e.g. Petri Dish)

27
Q

What is inoculation?

A

Introduction of a microbe into culture medium

28
Q

What is a pure culture?

A

Contains a single species of microbe

29
Q

What is a contaminated culture?

A

Contains an unwanted microbe or microbes

30
Q

What is the 4 genera of fungi?

A

Zygomycota, Ascomycota, Basidiomycota and Deuteromycota

31
Q

What is bacillus shape?

A

rod shaped bacteria

32
Q

What is cocci shape?

A

spherical shape bacteria

33
Q

What is spirillum shape?

A

Spiral shape bacteria

34
Q

What is strepto arrangement?

A

Chain like structure

35
Q

What is Staphylo arrangment?

A

cluster like “grapes”

36
Q

What is diplo arrangement?

A

Arranged by pairs

37
Q

What is tetra arrangement?

A

Arranged by fours

38
Q

When describing bacterial shape we will have to state the _____ and ________

A

Shape and arrangement

39
Q

What is general purpose media?

A

a media that will support the growth of a wide variety of bacteria
Con: microbes you dont want may be present

40
Q

What is selective media?

A

Grows certain selective types of microbes in one of two ways:
Providing or withholding certain nutrients to selectively grow microbes (e.g. PDA)
Adding inhibitory substances like antibiotics

41
Q

What is differential media

A

Addition of substances that cause bacteria to take on a different appearance from other species

42
Q

What is a smear?

A

thin layer of live specimen on a glass slide
Used for staining procedures
Smears can be made from either broth or agar culture

43
Q

How do you make a smear from agar?

A

First apply drop of water to slide with loop

Use inoculation needle to transfer microbes

44
Q

What is the advantage and disadvantage of agar smear?

A

Advantage: Relatively easy to control the amount of microbes to transfer
Disadvantage: Easy to add too much of the cultured microbe to the slide (result = smear will be too dense)

45
Q

How do you make a smear from broth?

A

Use inoculation loop for aseptic transfer

46
Q

What is the advantage and disadvantage of broth smear?

A

Advantage: Easier to make a smear from broth than from agar (one less step)
Disadvantage: Difficult to control amount of microbes transferred to slide

Microbiology 211 Lab (5 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions