Quiz 1 Flashcards
Label Parts of Microscope
Ocular lens, Objective Lens, Stage Light, course turning knob, fine turning knob
Name 2 organisms used in lab 2 and list 2 characteristics
B. megatherium: gram +, rod shaped
M. Letus: gram +, coccus
Two things you do after using a microscope:
- Wipe oil off lens
- Wrap up wire and unplug
Two reasons for using immersion oil when using the compound microscope under 100x
- improves resolution
- refracts light into objective lens
formula for total magnification
(mag of objective lens) x (mag of ocular lens)
difference between magnification and resolution?
- magnification enlarges or zooms into a microorganism
- resolution: allows you to differentiate between 2 organisms as distinct and separate
List 2 reasons why you need to stain microorganisms
-create a contrast with the background
differentiate between 2 groups of bacteria
Name and draw 3 diff types of bacterial cell morphologies
coccus (circle)
rod shaped (oval)
spirillium (wiggly oval)
Define simple stain
a single stain
What part of a petri dish should be labeled?
Name 3 things that you label
- the part with agar should be labeled
- Name of microorganism, your name, date of inoculation
When incubating a petri dish which part of the dish should be facing up?
Agar should be facing up during incubation
Know how to draw a T-streak. What is the goal of a Tstreak
to isolate a single colony of bacteria
Design an experiment to isolate S. marccescens
Gram stain both M. letus and B. megtaerium they will be purple since they are both gram +. S. marccescens is pink so it is gram -. You can take a piece of the pink and perform a t streak to isolate the colony after the bacteria grows.
