Quiz 1 Flashcards
3 TYPES OF BLOOD COLLECTION
- Venipuncture
- Capillary Puncture
- Arterial Puncture
most critical step in blood collection
Patient Identification
MT’s mortal sin
Mislabeling
must in phlebotomy
- Correct patient identification.
- Correct specimen identification. (Proper
labeling: Patient’s Full Name, Hospital
Identification Number, Location, Time and
Date and the initials of the phlebotomist) - Consistent with universal precautions
(Gloves, Gowns must be worn at all times
and hands must be washed in between
patients) - Aseptic Technique (Cleaning of puncture
site with 70% alcohol) - Sharp objects must be thrown into
appropriate containers and must not be
unsheathed or bent. - Gauze and cotton must be waste in
biohazard containers or trash bins.
VENIPUNCTURE
- Ideal procedure is to have the pa琀椀ent
lie down or if not possible, the patient should sit in a sturdy, comfortable chair and not on high stools.
There’s should be _____ inside the patient’s mouth during the procedure.
nothing
Ideal site for puncture:
Antecubital Fossa
Type of veins
Median Cubital Vein
Cephalic Vein
Basilic Vein
2 anatomical patterns
H pattern ans M pattern
H-Pattern
a. Median Cubital Vein
b. Cephalic Vein
c. Basilic
M-Pattern
a. Median Vein
b. Accessory Cephalic Vein
c. Basilic
are the
possible e昀昀ects of prolonged tourniquet applica琀椀on.
Hemoconcentra琀椀on, Hemolysis, Shortened Coagula琀椀on Time (PT/APTT)
Application of tourniquet must be _____ above puncture site.
3 – 4 inches
condition in which venous flow is slowed.
statis
is the most common needle size for adult.
21-gauge (1 inch long) needle
Phlebotomist must never puncture the patient _____.
twice
Patient should not _____.
pump the fist
Tourniquet Application must be less than
1 minute
Angle between skin and needle
Less than 30 degrees
Causes of Specimen Hemolysis
- Prolonged tourniquet application on
- Moisture or contamination of blood collecting tubes
- Needly with small bores
- Excessive agitation
- Frothing of the blood sample
Apply tourniquet _____ away from the site of the puncture.
2 – 4 inches
Apply ______ in a _____starting from inside then out.
antiseptics (70% alcohol) , circular manner
_______ manner can be done
Up and down or forward and backward
_____ cannot be used in capillary only
Betadine
Reapply tourniquet. Insert needle with _____.
15 – 30 degrees angle
Withdraw blood by pulling the _____, not too fast, not too slow.
plunger
Transfer blood ample to anticoagulated tube. Remove needle and tube cap and let blood flow on the sides to avoid ____.
hemolysis
ORDER OF DRAWS
Evacuated Tube and Syringe
- Yellow (Blood Culture Tubes)
- Blue Stopper (Coagulation Sodium Citrate Tube)
- Serum Tubes with or without clot activator or gel separator
- Green Stopper (Heparin Tubes w/ or w/o gel)
- Lavender Stopper (Ethylenediaminetetraacetic Acid Tubes)
- Gray Stopper (Glycolyctic Inhibitor Tubes)
Blood Culture Tube (Yellow) Number of Inversions
8
Citrated Tube (Light Blue Top) Number of Inversions
5
Serum Tube Red Top (Glass, Non additive) Number of Inversions
O
Serum Tube Red Top (Plastic, Clot Activator) Number of Inversions
5
Heparinized Tube (Green Top)
8
EDTA Tube (Lavender, Purple, Pink)
8
Sodium Fluoride Tube (Gray Top)
8
refers to the small blood vessels connecting the arterioles to the small veins
Capillary
Due to its ______, it is accessible only if small amount of blood is needed.
diameter
Capillary puncture is done if the patient is:
-Infants less than 1 year old.
-Severely burned patients
-Patients whose veins are reserved for therapeutic purposes.
-Extremely obese patients.
-Adult with poor veins.
Avoid applying ___,_____, ____
pressure, squeezing, “milking
Discard
first drop of blood
Discard excess —— and ————
tissue fluid and dead epidermal cells
Discard first drop of blood
Facilitate free flow of blood
Depth of Skin Puncture for Adult, Infants, Premature
o Adult: 2.0 – 2.5 mm
o Infants: <2.0 mm
o Premature: <0.85 mm
MATERIALS FOR CAPILLARY PUNCTURE
- Blood Lancet
- Cotton Balls
- 70% Alcohol
- Capillary Tubes
- Gauze
- Sealing Clay
SITES TO BE AVOIDED
- Hematoma
- Burned, damaged, occluded veins - Intravenous catheter (IV Line)
- Edema
- Post Mastectomy Side
- Skin with Tatto
COMPLICATIONS DURING VENIPUNCTURE
Ecchymosis (Bruise)
Hematoma
Fainting (Syncope)
Hemoconcentration
Intravenous (IV) Therapy
Mastectomy Patients
Obesity
Iatrogenic Anemia
Failure to Draw Blood
Petechiae
Nerve Damage
Hemolysis
Burned, Damage, Scarred and Occluded Veins Seizures and Tremors
Most common and Leakage of small amount of blood
Ecchymosis (Bruise)
Leakage of large amount of blood
Hematoma
Short lapse in consciousness
Fainting (syncope)
o Prolonged tourniquet applicationon
o Wait for 2 minutes before reapplying the tourniquet
Hemoconcentration
Wait for _____ before reapplying the tourniquet
2 minutes
o Draw in the opposite side of the IV
o Stop IV for 2 minutes and discard 昀椀rst 5-ml of blood
Intravenous (IV) Therapy
Draw in the _____ side of the IV
opposite
Stop IV for ___ minutes and discard first ____ of blood
2, 5mL
Draw blood from the opposite of mastectomy side
Mastectomy Patients
BP Cuff will help
Obesity
BP cuff will help (Must not be more than ____ and ____)
40 mmHg and 1 minute
is the study of blood cells and its components
Hematology
Red Blood Cells Medical Term
Erythrocytes
White Blood Cells Medical Term
Leukocytes
Platelets medical term
Thrombocytes
Human body is composed of approximately ______.
5 liters of blood
Red Blood Cells Components
▪ Hemoglobin
▪ Hematocrit
▪ Red Blood Cell Indices
▪ Reticulocytes
White Blood Cells Components
▪ Neutrophil
▪ Lymphocytes
▪ Monocytes
▪ Eosinophils
▪ Basophils
What to put in specimen identification
Patient’s first and last name
Hospital identification number
location
time and date
initials of the phlebotomist
Gauze and cotton waste must be placed in _____
biohazard containers
universal precautions
Gloves must be worn at all times, and hands must be washed in between patients
more
stable ; visible vein
Median Cubital Vein
not stable
Cephalic/ median cephalic vein
Basilic Vein
small ; not anchored well ; tendency to move ; close to brachial artery
light blue additive
3.2 sodium citrate
prevents blood from clotting by binding calcium
3.2 sodium citrate