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HEMATOLOGY LAB > Blood dilution > Flashcards

Blood dilution Flashcards

1
Q

reported as percentage

A

relative count

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2
Q

shows the real count/ number

A

absolute count

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3
Q
A
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4
Q

•Prior to blood cell counting using the ______, blood is first diluted with specific diluting fluids

A

Improved Neubauer counting chamber

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5
Q

RBc dilution if blood ratio

A

1:200

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6
Q

WBC dilution of blood ratio

A

1:20

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7
Q

PLT

A

1:100

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8
Q

Devices used first cell count

A

Thoma Pipettes, Improved Neubauer Hemacytometer, Compound microscope

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9
Q
A
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10
Q

RBC Pipet markings

A

0.5/1.0/101

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11
Q

WBC markings

A

0.5/1.0/11

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12
Q

RBc size of bulb

A

large

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13
Q

WBC size of bulb small

A

small

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14
Q

rbc color of bead

A

red

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15
Q

wbc color of bead

A

white

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16
Q

rbc volume in bulb

A

100

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17
Q

wbc volume in bulb

A

10

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18
Q

RBC size of bore

A

smaller

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19
Q

WBC size of bore

A

bigger

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20
Q

mark to which blood is drawn (RBC)

A

0.5 mark

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21
Q

mark to which blood is drawn (Plt count)

A

1 mark

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22
Q

the mark to which diluting fluid is drawn

A

101 mark

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23
Q

area to where both blood and diluting fluid combine

A

Bulb

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24
Q

mixes the contents; identifier of the type of thoma pipette

A

Red bead

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25
Q

to which diluting fluid is drawn (wbc)

A

11 mark

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26
Q

to which blood is drawn (wbc)

A

0.5 mark

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27
Q
A
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28
Q

Red blood cell count diluting fluids

A

Isotonic solutions: Hayem’s, Gower’s, or Dacie’s fluid.

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29
Q

WBC Count diluting fluids

A

2% acetic Acid; 1% Hydrochloric Acid; Turk’s Diluting fluid (Containing Acetic Acid-3ml, 1 ml of aqueous gentian violet, and 100 ml distilled water)

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30
Q

platelet count diluting fluid

A

•1% ammonium oxalate; Rees-Ecker fluid (Containing 3.8 grams of sodium citrate, 0.2 ml of 40% formaldehyde, 0.1 gram of brilliant cresyl blue in 100 ml aqueous solution)

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31
Q

DILUTING FLUIDS

A

• Cheap and Economical
• Stable, Easy to Secure and Prepare
• With Preservative Action
• High Specific Gravity
• With Buffer Action
• Non-Allergenic / Non-corrosive
• WBC Diluting Fluid must be hypotonic
• RBC Diluting Fluid must be Isotonic

32
Q

device used to count erythrocyte, leukocyte, and platelets, present in blood as well as other body fluids

A

hemacytometer

33
Q

Each chamber has a total measurement of

A

9mm² (Subdivided into 9 squares that measures 1mm² each)

35
Q

Refers to the routine procedure that gives an approximation of the total number of leukocytes in the circulation

36
Q

Function of the WBC is to

A

provide immunity- the ability to resist infection

37
Q

In a normal adult, WBC ranges about

A

4,000-11,000/mm3

38
Q

A Count above normal can is called

A

Leukocytosis

39
Q

leukocytosis PHYSIOLOGIC CAUSE

A
  • Exercise
  • Stress
  • Obstetric labor
  • Anesthesia
40
Q

leukocytosis parhologic causes

A

-Infection
-Hematologic Disorder (leukemia)

41
Q

counts below normal

A

Leukopenia

42
Q

Leukopenia causes

A

• Viral Infection
• ionizing Radiation
• Chemicals
• Drugs
• Hematologic problems (aplastic anemia)

43
Q

For RBC Counting, Blood is diluted with

A

isotonic solution

44
Q

WBC Dilution Ratio

45
Q

WBC Dilution Factor

46
Q

WBC Blood Units

47
Q

WBC Bulb Units

48
Q

Blood is diluted with either:

A

-2% acetic Acid
- 1% Hydrochloric Acid
-Turk’s Diluting fluid (containing acetic acid-3ml, 1
ml of aqueous gentian violet, and 100 ml distilled
water

49
Q

When WBC is markedly elevated (100-300 x10⁹/L)
the dilution can be increased

50
Q

When WBC is also below 3 x10⁹/L, the dilution can be
reduced to

51
Q

Difference between the total cells counted on each
side should agree

52
Q

are resistant to lysis during blood
dilution and are counted as WBC in the
hemacytometer

A

Nucleated RBC

53
Q

If more than ___ NRBC are seen in the blood smear
during differential count, the WBC count must be
corrected.

54
Q

Cells Touching the _____ must be
counted.

A

top and left lines

55
Q

Cells touching the _____ shall be
ignored.

A

bottom and right lines

56
Q

For RBC Counting, Blood is diluted with

A

isotonic
solution

57
Q

solution with equal concentration of
solutes within cells

A

isotonic solution

58
Q

RBC Diluting Fluids w/ components:

A

Hayem’s Fluid
Gower’s Fluid
Dacie’s Fluid

59
Q
  1. HAYEM’S FLUID
A

• Sodium Chloride(Provides isonicity) 1 g
• Sodium Sulfate (Prevents Rouleux) 5 g
• Mercuric chloride (antiseptic) 0.5 g
• Distilled Water (Solvent) 200 ml

60
Q

Provides isonicity

A

Sodium Chloride

61
Q

Prevents Rouleux

A

Sodium Sulfate

62
Q

antiseptic

A

Mercuric chloride

63
Q

Solvent

A

Distilled Water

64
Q
  1. DACIES’S FLUID
A

• Trisodium Citrate- 3.13 g
• Formaldehyde (37%) 1 ml
• Distilled water 100 ml

65
Q
  1. GOWER’S FLUID
A

• Isotonic solution containing 12.5 g of sodium sulfate
and 33.3 g of glacial acetic acid in 200 ml distilled
water

66
Q

• Isotonic solution containing 12.5 g of sodium sulfate
and 33.3 g of glacial acetic acid in 200 ml distilled
water

A

GOWER’S FLUID

67
Q

RBC Dilution ratio

68
Q

RBC Dilutionnfactor

69
Q

RBc Blood up to

70
Q

RBc fluid up to

71
Q

For patients with ____, increase the
dilution. For _____, reduce the dilution (1:100)

A

polycythemia

anemia

72
Q

are important in helping diagnose
bleeding disorders.

A

Platelet counts

73
Q

Platelets Function primarily in

A

hemostasis and maintaining capillary integrity

74
Q

Increased Platelet Count:

A

Thrombocytosis

75
Q

Decreased platelet count:

A

Thrombocytopenia

HEMATOLOGY LAB (4 decks)

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