Quiz 1

Question 1

Aseptic Technique Examples

Answer 1

• Using Sterile Gloves/Labcoats
• Flaming the Inoculation Loop
• Keeping Covers over TSA Plates

Question 2

Microliter (uL) to Milliliter (mL) comparison

Answer 2

Microliter: 10^-6 Liters

Milliliter: 10^-3 Liters

Question 3

Darkfield Microscopy

Answer 3

Used to view living, unstained microorganisms. Utilizes a darkfield condenser containing an opaque disc, only allowing light in that has been refracted by the specimen into the objective lens, which forms an image.

Used when analyzing thin and distinctly shaped bacteria

Question 4

Phase-Contrast Microscopy

Answer 4

Optimal for viewing living cells, examining bacterial motility and intracellular structures.

Exploits differences in refractive index between the cell cytoplasm and surrounding medium. A phase plate shifts light reflecting off of cells to form a sharp image of cells darker than the background

Question 5

Microscope Condenser

Answer 5

Gathers light waves from the microscope light source, directs them into a cone of light that illuminates the specimen

Question 6

Immersion Oil Function

Answer 6

Increases the resolution of a microscope by replacing the air gap between the immersion reflective lens and cover glass with a high refractive index medium (reducing light refration and leading to a more crisp picture)

Question 7

Relationship Between Amount of Light Needed and Magnification

Answer 7

The greater the magnification, the more light that is required

Question 8

Discerning True Motility from Brownian Movement

Answer 8

True motility will demonstrate actual movement of the cell, while a cell does not move in Brownian movement

Question 9

What is the evidence to support the claim that a microbe is capable of flagella-mediated movement?

Answer 9

Viewing under electron microscope to observe the rotation of the flagella which causes the cells to either move forward or reverse

Question 10

Serratia marcesens

Answer 10

Rod-shaped bacteria, colonies appear red

Question 11

Micrococcous luteus

Answer 11

Circular bacteria, colonies appear yellow

Question 12

Escherichia coli

Answer 12

Rod-shaped bacteria

Question 13

Saccharomyces cerevisiae

Answer 13

Yeast

Question 14

Rhodospirillum rubrum

Answer 14

Rod-shaped bacteria with flagellar movement

Question 15

Staphylococcus epidermidis

Answer 15

Circular, clumped bacteria that is not motile

Question 16

Peritrichous

Answer 16

Flagella cover cell

Question 17

Polar Lophotrichous

Answer 17

Multiple flagella on one pole

Question 18

Polar Monotrichous

Answer 18

Single flagella on pole

Question 19

Polar Amphitrichous

Answer 19

One flagella on each pole