Questions

Question 1

Which experiments give us the proteins concentration

Answer 1

1. Biuret (only for proteins concentration between 200-400 mg/l)
2. Folin’s method (only for proteins concentration between 200-500 mg/l)
3. refraction index- refractometer (depends on density of the material)

Question 2

Which has more eluted volume?

Answer 2

The small proteins

Question 3

In which methods will you use CuSO4?

Answer 3

Biuret

Folins

Question 4

In which method do we use the sulphosalicylic acid

Answer 4

Precipitation of proteins

Question 5

In which method do we use seignette salt?

Answer 5

In the quantity determination of proteins by Biuret reaction- Biuret reagent
& in In the quantity determination of proteins by Folins reagent

Question 6

In which method do we use ammonium sulphate

Answer 6

Salting out

Question 7

In which method do we use nesslers reagent

Answer 7

Dialysis

Question 8

Which methods are useful for the proteins separation

Answer 8

Salting out
Dialysis
Chromatography 
Gel filtration 
Electrophoresis
Western blot (blotting)

Question 9

What will be the color of the product in the Biuret reaction (for quality)

Answer 9

Violet if regular

If CuSO4 in excess blue

Question 10

What will be the color of the product in the folins method?

Answer 10

Blue color

Question 11

What will be the color of the NH4+ in the dialysis method

Answer 11

Yellow-brown

Question 12

In which method do we use the sodium bicarbonate (NaHCO3)?

Answer 12

Precipitation of proteins by acids