5.The estimation of protein concentration of blood plasma by determination of the refraction index

Question 1

what happens if a light beam reaches a medium with higher density?

Answer 1

it is bending towards the normal (α > β)

Question 2

what happens if a light beam reaches a medium with lower density?

Answer 2

light beam is bending away from the normal, (α < β).

Question 3

which line is called ‘normal’?

Answer 3

a line perpendicular to the surface, at the point of refraction

Question 4

the degree of the refraction is determined by?

Answer 4

the refraction indexes of the media, which are determined by the densities of the materials

Question 5

which refraction will be examined?

Answer 5

the second refraction will be examined: between the solution and the measuring prism.

Question 6

what does it mean the angle of refraction which belongs to the angle of incidence?

Answer 6

90°, is called the limiting angle (r).

Question 7

what are the needed materials for the portable refractometer?

Answer 7

 Blood plasma (concentrated)
 Abbe's refractometer
 Microscope lamp
 Glass rod
 Paper wadding

Question 8

The refraction index at given temperature only depends on

Answer 8

The density