Questions Flashcards
You are performing an experiment where you would like to analyse DNA molecules in the size range 500 kilobases-1megabase. Which type of procedure would be most suitable?
A. Standard agarose gel electrophoresis
B. Standard polyacrylamide gel electrophoresis
C. Pulse gel field electrophoresis
C. Pulse field electrophoresis.
Separates depending on size, and can operate above the kilobase range
During SSCP experiments, nucleotide substitutions, such as mutations in single stranded DNAs causes them to migrate at different rates because the nucleotide substitutions…
A. significantly alter the molecular weight of the ssDNA.
B. result in the ssDNA taking on a different shape.
B. They result in the ssDNA taking on a different shape.
You would like to investigate the suspected presence of a point mutation in a patient’s DNA sample and decide to start your investigation with an SSCP experiment. What electrophoresis conditions would you employ during the experiment?
A. Denaturing conditions
B. Non-denaturing conditions
B. Non-denaturing conditions.
Denaturing conditions would prevent the ssDNA from forming secondary structures which would reveal the presence of a point mutation.
During a size determination analysis, which two of the following are you most likely to add formamide to?
A. Single stranded DNA
B. Double stranded DNA
C. Single stranded RNA
D. Double stranded RNA
A. Single stranded DNA
C. Single stranded RNA
Formamide is used to denature single stranded nucleic acids. Double strands are held together by Watson-Crick interactions, so don’t form interactions that can be denatured. Whereas single stranded nucleic acids must be linearised.
During a southern blot procedure, whic of these elements oif the technique enables you to most definitely assess the presence of a DNA species of known size?
A. A size marker ladder electrophoresed alongside your sample.
B. A labeled oligonucleotide
B. A labeled oligonucleotide.
Knowing the size isn’t definitive proof, whereas the oligonucleotide would be specific to the DNA species.
How to tell if something is an expression vector?
Expression vectors have a promoter (usually CMV) upsteam of a GFP.
You would like to introduce a mutated gene of interest into a mammalian cell line and be able to study the functional effects over the course of several months. Which of these techniques would be most suitable?
A. Heat shock.
B. Lipofection.
C. Retrovirus mediated transfection.
C. Retrovirus mediated transfection
Enables the DNA of interest to integrate into the host genome, maing it stable and protecting it from nucleases.
The aim of your mouse Cre-LoxP engineering project is to “knock-out” a gene of interest specifically in the liver. Which one of these components would be practically relevant to your transgenics workflow?
A. Insertion of LoxP sites flanking the gene of interest specifically in the liver
B. Insertion of a Cre recominase gene into thte genome specifically in the liver.
C. A Cre recombinase gene under the control of a promoter that is specifically activated in the liver.
C. A Cre recombinase gene under the control of a promoter that is specifically activated in the liver.
Actually targets the liver as opposed to B, which isn’t specific. A is nice in theory, but must be very difficult to actually achieve, especially without a cre.
Which of the following is not true of integrin?
- Intracellular signals can activate integrins causing them to reach out and grab hold of extracellular structures.
- Integrin molecules serve as stable, permanent anchors that anchor a cell to the extracellular matrix.
- When integrin binds to the extracellular matrix, it stretches into an extended, activated state to attach to molecules on the inside of the cell.
- Integrins undergo extensive conformational changes on binding to molecules on either side of the plasma membrane.
2) Integrin molecules serve as stable, permanent anchors that anchor a cell to the extracellular matrix.
Which of the following determines the direction that cellulose microfibrils are laid down in the extracellular space of a plant cell?
- Amount of turgor pressure within a cell
- Orientation of microtubules on the cytoplasmic side of the plasma membrane
- Orientation of microtubules in the cell wall
- Availability of sugar monomers for cellulose microfibril elongation
- Orientation of cellulite on the cytoplasmic side of the plasma membrane
2) Orientation of microtubules on the cytoplasmic side of the plasma membrane
Microtubules don’t exist in the cell wall, only cytoplasmically.
Not 1 because it would be equal throughout the cell.
Not 4 because sugars would probably only effect the rate, not direction.
Which of the following techniques could be used to determine relative specific transcript levels between RNA samples?
1. Southern blotting
2. Western blotting
3. Eastern blotting
4. Northern blotting
4) Northern blotting
Analyses RNA in similar way to southern.
Which of the following would be expected to be essentially the same?
- cDNA libraries made from mouse liver and kidney cells.
- cDNA and genomic libraries made from mouse liver cells.
- genomic libraries made from mouse liver and kidney cells.
- cDNA and genomic libraries made from mouse kidney cells.
3) Genomic libraries made from mouse liver and kidney cells.
The genome will be the same
Not 1 cDNA is made from mRNA using reverse transcription – so different for different cell types.
Which of the following define the barrier between apical and basolateral surfaces of mammalian epithelial cells?
- Tight Junctions
- Gap Junctions
- Desmosomes
- Hemidesmosomes
- Adherens junctions
1) Tight Junctions
Which of the following is inconsistent with the function of gap junctions?
- They allow ATP-driven pumps to move substances between attached cells.
- They allow an electrical and a metabolic coupling between attached cells.
- They allow the cytoplasm of two adjacent cells to be continuous with each other.
- They allow inorganic ions to move directly between attached cells.
- They can open or close as needed.
1) They allow ATP-driven pumps to move substances between attached cells.
They are just holes, no pumps here.
Abnormally stretchy skin is part of a genetic syndrome that could result from which of the following?
- Increased production of collagen
- Loss of proteinase that cleaves procollagen.
- Hypersecretion of procollagen
- Synthesis of excess cellulose
- Overactivity of proteinase that cleaves procollagen.
2) Loss of proteinase that cleaves procollagen.
Would reduce the amount of collagen.
RTKs can activate the enzyme phosphoinositide 3-kinase, which phosphorylates inositol phospholipids. These phospholipids then do what?
- Activate Ras.
- Serve as phosphate donors in phosphorylation reactions.
- Serve as docking sites that recruit specific intracellular signalling proteins to the plasma membrane.
- Activate Ca2+ channels in the plasma membrane, promoting an influx of Ca2+ into the cytosol.
- Activate G proteins.
3) Serve as docking sites that recruit specific intracellular signalling proteins to the plasma membrane.
Targeted chemotherapies can alter intracellular signaling pathway at steps downstream of the EGFR. Which potential treatments could be effective for treating tumors like glioblastoma caused by overactivation of EGFR?
- Addition of a drug that inhibits serine and threonine kinase activity.
- Addition of a drug that blocks the phosphorylation sites on AKT.
- Addition of a drug that inhibits Ras GEF function.
- Addition of a drug that inhibits Ras GAP function.
- Addition of a drug that inhibits serine and threonine kinase activity.
- Addition of a drug that blocks the phosphorylation sites on AKT.
- Addition of a drug that inhibits Ras GEF function.
Inhibiting Ras GAP function could lead to the proliferation of cells.
The anaphase-promoting complex or cyclosome (APC/C) triggers the onset of anaphase by doing which of the following?
- Triggering the destruction of the condensins that hold the duplicated chromosomes in a condensed state.
- Phosphorylating the motor proteins that pull the spindle poles apart
- Triggering the destruction of the cohesins that hold the sister chromatids together.
- Cleaving spindle microtubules
3) Triggering the destruction of the cohesins that hold the sister chromatids together.
Anaphase is where the sister chromatids move.
Different cyclin-dependent protein kinases (Cdks) trigger different stages of the cell cycle in part because of which reason(s)?
- Their concentrations increase at different stages of the cycle.
- They are degraded at different stages of the cycle.
- Their activities increase at different stages of the cycle.
- All of the above are correct about the cyclin-dependent kinases.
3) Their activities increase at different stages of the cycle.
Because of the cyclin.