Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Histotech Exam 4 > Question Manuel > Flashcards

Question Manuel Flashcards

1
Q

Glycogen is best demonstrated by the use of:
Crystal violet colloidal iron
Mayer mucicarmine
Periodic acid-Schiff (PAS) with and without diastase
Alcian blue with and without Hyalaronidase

A

Periodic acid-Schiff (PAS) with and without diastase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Amyloid can be demonstrated with
Congo red
Mayer mucicarmine
Crésyl echt violet
Alcian bleu

A

Congo red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

The Schiff réaction demonstrates:
Hyalaronidase acid
Aldehydes
Basophiles compounds
Amphoteric tissus components

A

Aldehydes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

A good controle for the Mayer mucicarmine stain is:
Kidney
Appendix
Spleen
Liver

A

Appendix

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

À good controle for glycogen is:
Kidney
Appendix
Lung
Liver

A

Liver

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

To increase the specificity for amyloid, Congo red stains should be examined by which of the following types of microscopy

Phase contrast
Dark field
Polarized
Electron

A

Polarized

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Of the following, which is the best fixative for glycogen
gluteraldehyde
Orth
acetic acid
absolute alcohol

A

Absolute alcohol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Periodic acid uis used in the PAS technique as a/an:
Mordant
Stain
Reducer
Oxidizer

A

Oxidizer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Acid mucopolysaccharides are demonstrated by:
Alcian bleu
Alizarin red
Congo red
Acid fuchsin

A

Alcian bleu

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

The reliability of the Schiff reagent may be checked by adding which of the following to a small aliquot of Schiff solution
Sodium iodate
Formaldehydes
Potassium Métabisulfite
Diastase

A

Formaldéhyde

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

A fluorescent dye used for the demonstration of amyloid is:
Crystal violet colloidal iron
Auramine O
Colloidal iron
Thioflavin T

A

Thioflavin T

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

The alcian blue stain performand at pH 1,0 demonstrates:
All acid mucopolysaccharides
Carboxylated acid mucopolysaccharides
Sulfatés acid mucopolysaccharides
Neutral mucopolysaccharides

A

Sulfatés acid mucopolysaccharides

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Substance stained positive with the colloidal iron procedure will be:
Red
Blue
Apple green
Violet

A

Blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Schiff reagent is a/an
Reduced solution of basic fuchsin
Oxidized solution of basic fuchsin
Reduced solution of acid fuchsin
Oxidized solution of acid fuchsin

A

Reduced solution of basic fuchsin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Diastase digestion increased specificity for
Mucin
Amyloid
Carbohydrate
Glycogen

A

Glycogen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Colloïdal iron is used for the demonstration of
Amyloid
Glycogen
Acid mucopolysaccharides
Neutral mucopolysaccharides

A

Acid mucopolysaccharides

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Glycogen may be removed from tissue by digestion with
A-amylase
Hyluronidase
Sialic acid
Chitin

A

A-amylase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Adjacent sections are stained with PAS, one with and one without diastase digestion. A positive result on the one without digestion and a negative result on the one with digestion indicates the presence of:
Carboxylated mucosubstances
Sulfatés mucosubstances
Hyalaronic acid
Glycogen

A

Glycogen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Which of the following fungi are well demonstrated with the colloidal iron stain?
Histoplasma capsulatum
Cryptococcus neoformans
Candida ablicans
Coccidioides immitis

A

Cryptococcus neoformans

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

True or false
Glucose, sucrose and other oligopolysaccharides can be demonstrated easily in tissue sections

A

False

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

True or false
Hyalaronidase is used to digest some connective tissue mucin

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

True or false
The routine alcian blue stain is done at a pH of 1,5

A

False

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Good Schiff reagent should be light pink
True or false

A

False

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Amyloid shows a yellow biréfringence following staining with Congo red
True or false

A

False

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Glutaraldéhyde is one of the recommended fixatives for the PAS reaction
True or false

A

False

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

The Schiff reaction may show false positivity following chromate-containing fixatives
True or false

A

False

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

True or false
Glycogen containing tissue fixed with Bouin solution may show resistance to a diastase digestion

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

No staining of the glomérular basement membrane can be seen microscopically on a control sections of kidney. This may be a result of:
Inadequate oxidation
Prolonged treatment with Schiff reagent
Thickening of the basement membrane
Lack of treatment with sodium Métabisulfite

A

Inadequate oxidation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

Marked nonspecific background staining is noted on a section with the PAS technique. This could be a result of:
Over oxidation of the section
Prolonged washing with water
Fixation with glutaraldéhyde
Fixation with Zenker solution

A

Fixation with glutaraldéhyde

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

Very weak staining is noted on a PAS-staining control sections of liver. One problem solving action is:
Check if Schiff reagent with formaldehyde
Verify the use of potassium Métabisulfite
Shorten the oxidation time
Decrease the washing time

A

Check if Schiff reagent with formaldehyde

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

Sections of small intestine show orange goblet cells that are partially obscured by the yellow background. This is likely the result of:
Prolonged staining with mucicarmine
Overstraining with metanil yellow
Improper use of iron Hématoxyline
A port choice of control tissue

A

Overstraining with metanil yellow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

Control sections stained with Congo red show only yellow and no green, biréfringence. This could probably be corrected in the future by
Reducing the time in the Congo red show
Using iron Hématoxylin for the nuclear stain
Ensuring that the sections are cue at 8-10 um
Using only control tissue fixed in formalin

A

Ensuring that the sections are cue at 8-10 um

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

With both the Masson and Gomori trichrome procedures, Muscle satins:
Red
Green
Blue
Yellow

A

Red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

Which of the following méthodes best demonstrates elastic tissue
Verhoeff-van Gieson
Silver imprégnation
Gomori thrichrome
Periodic acid-Schiff (PAS)

A

Verhoeff-van Gieson

35
Q

The first step in most réticuline methods is
Silver imprégnation
Sensitization
Reduction
Oxidation

36
Q

The Verhoeff method differentiates with
An acid
A base
An oxidizer
Excess mordant

A

Excess mordant

37
Q

Van Gieson solution stains collagène
Green
Blue
Red
Yellow

38
Q

Bouin solutions functions in the Masson trichrome stain as a/an:
Oxidizer
Decolorizer
Reducer
Mordant

39
Q

The components of van Gieson solution are:
Picric acid and basic fuchsin
Picric acid and acid fuchsin
Aniline blue and Biebrich scarlet
Light green and acid fuchsin

A

Picric acid and acid fuchsin

40
Q

Most silver stains use which of the following as toning agent?
Sodium Thiosulafte
Formaldéhyde
Uranyl nitrate
Gold chloride

A

Gold chloride

41
Q

The reducing agent in most réticulin stains is
Formaldehyde
Periodic acid
Uranyl nitrate
Sodium Thiosulafte

A

Formaldehyde

42
Q

A good control for reticulin stains is
Kidney
Blood vessel
Liver
Skin

43
Q

Toluidine blue is used to demonstrate which of the following cells?
Plasma
Mast
Fibroblasts
Macrophage

44
Q

The component of basement membranes that is usually demonstrated with special stains is:
Protein
Carbohydrate
Laminin
Collagen

A

Carbohydrate

45
Q

À stain that might be used to demonstrate cirrhosis of the liver is the
Orcein
Verhoeff-van Gieson
Masson trichrome
Méthénamine silver

A

Masson trichrome

46
Q

In the Masson trichrome, Biebrich scarlet is removed from collagen by:
Acetic acid
Aniline blue
Picric acid
Phosphotungstic acid

A

Phosphotungstic acid

47
Q

Iron Hématoxylin, rather than aluminium hemtoxylin, is usually used to satin nuclei in trichrome procedure because
Subsequent staining solutions are acidic
Of the staining solution stability
The satin is more easily prepared
The mounted sections are more permanent

A

Subsequent staining solutions are acidic

48
Q

The preferred fixative for the Masson trichrome stain is:
Bouin solution
10% neutral buffered formalin
Glutaraldéhyde
Absolute alcohol

A

Bouin solution

49
Q

Silver imprégnation stains for reticulin depend on the formation of which of the following chemical groups?
Quinoid
Aldehyde
Carboxyl
Amino

50
Q

Sections for the demonstration of basement membranes should be cut at
2
4
6
6

51
Q

The oil red O stain requires which of the following sections:
Paraffin
Celloidin
Frozen
Plastic

52
Q

The oil red O stain might be used to demonstrate:
rhabdomyosarcomas
leiomyosarcomas
liposarcomas
adenocarcinomas

A

liposarcomas

53
Q

Which of the following methods is an exemple of physical staining?
Toluidine blue
Verhoeff-van Gieson
Méthénamine silver
Oil red O

A

Méthénamine silver

54
Q

Fat is chemically fixed and maintained in tissue by:
Osmium tetroxide
Mercuric chloride
Calcium formol
Carnoy solution

A

Osmium tetroxide

55
Q

Plasma cells can be demonstrated with
Methyl green-pyronin (MGP)
Verhoeff-van Gieson
Toluidine blue
PAS

A

Methyl green-pyronin (MGP)

56
Q

When ferric ammonium sulfate is used in a silver stain for reticulin, it functions as the
Oxidizer
Sensitizer
Reducer
Toner

A

Sensitizer

57
Q

When used in a silver stain for reticulin, phosphomolybdic acid functions as the
Oxidizer
Sensitizer
Reducer
Toner

58
Q

The Verhoeff Van Gieson shows both orange collagen and orange muscle. This most likely could be corrected in the future by
Différentiating longer in 95% alcool
Ensuring that the Picric acid solution is saturated
Increasing the concentration of the acid fuchsin
Mordanting the section in Bouin solution

A

Ensuring that the Picric acid solution is saturated

59
Q

The Masson trichrome stain shows only faint grayish pink staining of the muscle. This could most likely be prevented in the future by
Increasing the concentration of phosphotungstic acid
Using a mixture of phosphotungstic and phosphomolybdic acid
Using fresh acid fuchsin-Biebrich scarlet solution
Ensuring that the sections are acidic at the end of the stain

A

Using fresh acid fuchsin-Biebrich scarlet solution

60
Q

Fat droplets are seen in the tissue space of an oil red P-staioned section. This most likely resulted from
Dissolving the satin in propylene glycol
Using glycerine jelly for mounting the sections
Pressing on the cover glass to remove air bubbles
A displacement of fat during paraffin processing

A

Pressing on the cover glass to remove air bubbles

61
Q

Formalin fixed tissue shows very faded blue staining with the Masson thrichrome technique. The most likely explanation is that the sections were
Left too long in the final acetic acid solution
Stained with old acid fuchsin
Fixed in Bouin solution
Dried in a hot dryer

A

Left too long in the final acetic acid solution

62
Q

Acid fast stains ,at best negative if the tissue was fixed in:
10% formalin
B-5 solution
Carnoy solution
Zenker solution

A

Carnoy solution

63
Q

In the fite méthode, the organisms stain:
Red
Blue
Green
Black

64
Q

After Schiff reagent, tissues are rinsed in a sulfite solution to:
Fix the stain in the tissue
Différentiate the stain
Remove the excess leucofuchsin
Oxidize the tissue

A

Remove the excess leucofuchsin

65
Q

The property on which the acid fast stains depends is its:
Affinity for anionic dyes
Capability for reducing silver
Failure to satin with a dye other than basic fuchsin
Ability to resist decolorixation with dilute acids

A

Ability to resist decolorixation with dilute acids

66
Q

Which of the following is a method for demonstration of fungi
Ziehl Nielsen
Warthin-starry
Giemsa
Periodic acid-Schiff (PAS)

A

Periodic acid-Schiff (PAS)

67
Q

The best satin for the demonstration of mycobacterium leprae is the
Fite
PAS
Kinyoun
Gram

68
Q

Artifactual precipitate seen in the Groccott stain may be the result of using
Plastic forceps
Gelatin in the water bath
Glassware that was not chemically cleaned
Excess time in the gold chloride solution

A

Glassware that was not chemically cleaned

69
Q

Spherical or avoid bacteria are classified as
Rickettsia
Bacilli
Spirochètes
Cocci

70
Q

The carbol-fuchsin methods are specific for
Mycobacteria
Dimorphic fungi
Diplococci
Spore-forming bacilli

A

Mycobacteria

71
Q

Carbol-fuchsin contains
Acid fuchsin and HCL
Acid fuchsin and phenol
Basic fuchsin and HCL
Basic fuchsin and phenol

A

Basic fuchsin and phenol

72
Q

The term «mycosis» is used to describe a disease caused by
Fungi
Viruses
Bacteria
Rickettsiae

73
Q

Stains for the demonstration of spirochètes are based on their property of
Argentophilia
Argyrophilia
Metachromasia
Acid-fastness

A

Argyrophilia

74
Q

Which of the following is a Méthénamine silver technique
Ziehl Nielsen
Grocott
CAS
Brown & brenn

75
Q

The PAS reaction will demonstrate fungi because the cell wall contains
Lipids
Carbohydrates
Reducing substances
Argyrophilic protein

A

Carbohydrate

76
Q

The preferred fixative for the Warthin Starry technique is
Carnoy
Zenker
B-5
10% buffered formalin

A

10% buffered formalin

77
Q

The developper in the Warthin Starry stain is
Gelatin
Hydroquinone
Pyridine
Uranyl nitrate

A

Hydroquinone

78
Q

If fungi are to be demonstrated, a good counter stain for the PAS technique is
Nuclear fast red
Van Gieson
Light green
Eosin

A

Van Gieson

79
Q

An antigen is a substance that triggers the production of:
Immunogens
Immunoglobulins
Hormones
Epitopes

A

Immunoglobuline

80
Q

A light chain present in some antibodies is
IgM
IgG
IgE
K

81
Q

Différent molecular sites on antigens are known as
Antibody classes
Heavy chains
Epitopes
Immunoglobuline

82
Q

When an antibody labelled with a chromogen reacts with tissue from a patient, the immunohistochemical technique is called
Direct
Indirect
Avidin-biotin complex
Soluble enzyme immune complex

83
Q

Which of the following is a flurochrome
Alkaline phosphatase
Rhodamine
Fast red
Diaminobenzine

Histotech Exam 4 (16 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions