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Biochemistry > Qualitative Tests For Carbohydrates > Flashcards

Qualitative Tests For Carbohydrates Flashcards

1
Q

Molisch

A

General test for CHO
Alpha- napthol + sulfuric acid
Violet ring at the junction

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2
Q

Anthrone

A

General test for CHO
Anthrone
Green color

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3
Q

Fehling’s

A

Reducing sugar
Copper sulfate and Na-K tartrate
Brick red ppt

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4
Q

Benedict’s

A

Reducing sugar
Copper sulfate + sodium carbonate + sodium citrate
Brick red ppt

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5
Q

Barfoed’s

A

Reducing sugar (differentiates mono and di)
Copper sulfate + acetic acid
Brick red ppt (<5 mins for mono; >15 mins for di)

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6
Q

Tollen’s

A

Reducing sugar
Ammoniacal silver nitrate
Silver mirror

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7
Q

Mallard’s

A

Reducing sugar
Amino acids
Brown color

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8
Q

Seliwanoff’s

A

Ketoses
Resorcinol, HCl
Pink/ cherry red

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9
Q

Bial’s

A

Pentoses
Orcinol, HCl
Blue green color

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10
Q

Tollen’s Phloroglucinol

A

Pentoses
Phloroglucinol
Red color

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11
Q

Iodine

A

Starch, glycogen
Iodine
Starch: blue
Glycogen: red

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12
Q

Mucic acid

A

Nitric acid
Galactose
White insoluble crystals (galactaric/ mucic acid

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13
Q

Osazone/ Kowarsky

A

ID of sugars
Phenylhydrazine
Yellow orange crystals of different shapes

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14
Q

Osazone/ Kowarsky for glucose

A

Needle shaped

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15
Q

Osazone/ Kowarsky for fructose

A

Needle shaped

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16
Q

Osazone/ Kowarsky for mannose

A

Needle shaped

17
Q

Osazone/ Kowarsky for galactose

A

Balls with thorny edged shape
Cotton puff

18
Q

Osazone/ Kowarsky for xylose

A

Fine long needle shaped

19
Q

Osazone/ Kowarsky for maltose

A

Sunflower shaped

Biochemistry (5 decks)

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