QF-PCR Flashcards
QF PCR amplifies markers and provides a
Rapid analysis.
QF -
Quantitive fluorescence.
QF PCR has the addition of a
DNA probe which is like a primer.
The DNA probe has a …
Reporter and a quencher.
The reporter is a
Fluorescent tag
The quencher absorbs light form the reporter when
It is in close proximity. Only a few bases apart.
The DNA probe bonds to the target DNA at the same time as the primer, during the
Annealing stage.
If the sample is negative and the DNA doesn’t bond then neither will the probe, the reporter and quencher….
Stay in close proximity and no light is emitted.
During elongation, when taq reached the probe, it….
Dissembles the strand and replaces all the nucleotides.
After taq reaches the probe, the quencher and reporter are separated, therefore…
The quencher no longer stops the reporter from emitting light.
The emitted light is a
Released fluorophore.
The emitted light is monitored by….
Shining light of a specific wavelength.
Four main uses…
Genetic engineering.
Forensics and crime scenes.
Archeology.
Diagnosing disease.
Can be Quantitive by using…
Controls of known amounts that are compared to the sample.
