Q2-FUN15+16/Methods of Molecular Biology I and II Flashcards
Explain gel electrophoresis. and western blotting
Sometimes genetic diesease can be caused by mutations in a single gene. These mutations can be found using gel electrophoresis.
https://www.osmosis.org/learn/Gel_electrophoresis_and_genetic_testing
What is the first step in gel electrophoresis?
DNA is chopped up into small fragments by restriction enzymes
What are restriction enzymes?
What happens after the DNA is cut up by restriction enzymes in gel electrophoresis?
the DNA fragments are poured into a well within a piece of agarose gel.
There are charges placed on each side of the gel. Which one is negative and which one is positive?
When an electric current is passed through the gel, what happens?
the negatively charged DNA fragments move towards the positive end
When an electric current is running through the gel, the smaller fragments can move _________ than the larger fragments.
faster
What DNA sequence does EcoR1 bind to?
GAATTC
Explain western blotting.
Explain polymerase chain reactions.
Where does EcoR1 break the GAATTC sequence?
G | AATTC, between the G and first A
