Protozoans Flashcards
2 types of parasite
Protozoa (unicellular) and Metozoa (multicellular)
Types of Protozoa
Sarcondina (amoebas)
Mastigapora (flagellates)
Cilliata (ciliates)
Sporozoa
Types of Metozoa
Platyhelminthes
Nemahelminthes
Types of Platyhelminthes
Cestodes (tapeworm)
Trematodes (flukes)
Single celled eukaryotes.
No cell wall, instead periplast (cell-wall like).
Contains 1 or several nuclei.
PROTOZOA
Endoplasm contain food preserve, food vacuoles, contractile vacuoles, and chromatoidal bodies.
PROTOZOA
With special organs for locomotion: pseudopodia, cilia, undulating membrane or flagella.
PROTOZOA
Both free living and parasitic.
PROTOZOA
Protozoa 2 regions of the cytoplasm
ectoplasm ( outer) and endoplasm (inner).
Organ of Locomotion for Sarcodina (amoeba)
Pseudopods
Organ of Locomotion for Mastigapora (flagellates)
Flagella
Organ of Locomotion for Ciliata (ciliates)
Cilia
Organ of Locomotion for Sporozoa
None
Use pseudopodia for locomotion.
CLASS RHIZOPODA (amoeba)
the only pathogenic amoeba.
Entamoeba Histolytica
Motile. Can be progressive or non-progressive.
Non infective stage.
Feeding and vegetative stage.
Found in diarrhea and liquid stool.
Can be destroyed by iodine.
Encystation: trophozoite to cyst formation at the large intestine.
The endplasm may contain rbc, bacteria or vacuoles
Tropozoite
amoeba that does not reside the large intestine
Entamoeba Gingivalis
Ovoid or spherical shape.
Non-motile stage.
Cytoplasm has presence of chromatoidal bodies.
Non feeding stage, infective stage.
Found in formed stools.
Excystation: cyst to trophozoite formation at the small intestine.
Cyst
Types of Rhyzopodia Amoebas
Entamoeba Histolytica
Entamoeba Coli
Entamoeba Gingivalis
Entamoeba Hartmanni
Iodoamoeba Butschlii
Endolimax Nana
Diseases linked with Entamoeba Histolytica
Amoebiasis,
Amoebic dysentery,
Amoebic hepatitis
Other names for Entamoeba Histolytica
Amoeba coli,
Entamoeba dispan,
Entamoeba dysenteriae,
Amoeba dysenteriae.
Progressive/directional motion.
Finger like dendritic pseudopodia
Endoplasm contains RBC’s but no bacteria
Nucleus: thin nuclear membrane with layer of uniformed sized fine chromatin granules
Karyosome: fine centrally located ( bull’s eye)
Entamoeba Histolytica (Trophozoite)
Centrally located karyosome
Coffin shaped or sausage shape chromatoidal bars
Contains 4 nuclei.
Remains viable for 12 days in moist cool environment and 9-30 days in water.
Entamoeba Histolytica (Cyst)
Pathogenic which can invade tissues
large race E. Histolytica
Sign and symptoms:
Abdominal tenderness with diarrhea to dysentery which lasted for several days.
Prolong infection causes excessive lost of water and electrolytes that result to damage to large intestine.
Peritonitis occurs when intestinal walls is perforated due to bacterial invasion.
Bleeding due to ulceration of colon.
Pathogenesis (E. Histolytica.)
Non-pathogenic-____ E. Histolytica.
small race E. Histolytica.
Inflammation of the liver due to extraintestinal invasion of E. Histolytica. Clinical presentations are hepatomegaly, fever and leucocytosis.
Amoebic hepatitis-
Most common in tropic and sub-tropic areas example Philippines.
Amoebic hepatitis
Chromatoidal bodies resemble rice grain-shape or thin fan-like.
Eccentric karyosome.
ENTAMOEBA HARTMANNI
Small race E. histolytica
Trophozoite does not ingest RBCs
Causes only mild abdominal infection to human.
ENTAMOEBA HARTMANNI
Other name for ENTAMOEBA HARTMANNI
E. minuta,
E. minutissima
Other name for ENTAMOEBA COLI
Amoeba coli,
Endamoeba hominis,
Laschia coli.
Moves at different direction/ sluggish motion
Several small pseudopods.
Endoplasm contains bacteria and food vacuoles.
ENTAMOEBA COLI (trophozoite)
Nucleus consist of thicker nuclear membrane with layer of variously sized chromatin granules unevenly distributed.
Large eccentrically located nucleus.
ENTAMOEBA COLI (trophozoite)
Eccentric karyosome.
Chromatoidal bars is like splinter-like needles “witch broomstick”.
Contains 8 nuclei
ENTAMOEBA COLI (cyst)
Resides in the large intestine.
Smallest amoeba ( RBC in size).
Sluggish movement.
ENDOLIMAX NANA
Karyosome: large round dot.
Cyst: oval and contains 4 nuclei.
Comma shaped chromatoidal bodies
ENDOLIMAX NANA
Other name for ENDOLIMAX NANA
Entamoeba nana,
Endolimax intestinalis
Other name for IODAMOEBA BUTSCHLII
E. williamsi,
E. butschlii,
Iodine cyst,
Endolimax. williamsi
Progressive direction
Karyosome: large irregular and rounded with a cluster of acromatic granules
IODAMOEBA BUTSCHLII
Inhabits the intestine of human and pigs.
Cyst contain glycogen vacuoles appears a golden-brown by D’ Antoni’s.
Non-pathogenic.
IODAMOEBA BUTSCHLII
Other name for ENTAMOEBA GINGIVALIS
A. gingivalis,
A. buccalis,
E. buccalis
Found in the mouth
Pseudopodia is the same with E. Histolytica but does not exhibit progressive movement.
ENTAMOEBA GINGIVALIS
Small and centrally located karyosome.
Cytoplasm contains food vacuole with leukocytes and bacteria.
No cystic stage.
Transmitted through kissing, droplets, contaminated utensils.
ENTAMOEBA GINGIVALIS