Protozoans

Question 1

Characterized by the presence of hyaline foot-like extrusions from the ectoplasm (for movement)

Answer 1

Amoeba

Question 2

Old Phylum classification of Amoeba

Answer 2

Phylum Sarcomastigophora

Question 3

4 stages in the life cycle of amoeba

Answer 3

1. trophozoite
2. pre-cystic stage
3. cystic stage
4. metacystic stage

Question 4

feeding from/labile; motile, active, recovered in fresh samples

Answer 4

trophozoite

Question 5

uni-nucleated; starts to become a cyst

Answer 5

pre-cystic stage

Question 6

infective stage, non-feeding stage, resistant to environment, non-motile

Answer 6

cystic stage

Question 7

cyst starting to become a trophozoite

Answer 7

metacystic stage

Question 8

process when trophozoite transforms into a cyst

Answer 8

Encystation

Question 9

process when a cyst transforms into a trophozoite

Answer 9

Excystation

Question 10

Common in people who live in tropical areas with poor sanitary conditions

Answer 10

Entamoeba histolytica

Question 11

Most pathogenic amoeba

Answer 11

Entamoeba histolytica

Question 12

Habitat of E. histolytica

Answer 12

large intestines

Question 13

How many nucleus does a mature E. histolytica cyst have?

Answer 13

4

Question 14

What does the E. histolytica trophozoite release which causes lysis and necrosis in the tissue?

Answer 14

Lectin, amoeba pores, cysteine proteinases

Question 15

Appearance of E. histolytica cyst

Answer 15

round with thin cell wall, karyosome centrally located, cigar shape/sausage shape chromatoidal bar; size=10-12um (12-15um)

Question 16

Motility of E. histolytica trophozoite

Answer 16

progressive/aggressive/rapid directional

Question 17

What does hematophagia indicate?

Answer 17

presence of RBC; trophozoite is in its feeding form

Question 18

Size of E. histolytica trophozoite

Answer 18

12-60 um (15-20 um)

Question 19

Cytoplasm of E. histolytica trophozoite

Answer 19

finely granular, “ground-glass apearance”

Question 20

Parasites that may appear similar to E. histolytica

Answer 20

E. dispar and E. moshkovskii

Question 21

Non-pathogenic lumen dweller that is transmitted through ingestion of cyst from contaminated water or food

Answer 21

Entamoeba coli

Question 22

Appearance of E. coli cyst

Answer 22

spherical/oval; octo-nucleated with eccentric and large karyosom with peripheral chromatin and splinter shaped chromotoidal body with rough, pointed ends

Question 23

Size of E. coli cyst

Answer 23

10-35 um (12-25 um)

Question 24

Other 3 intestinal protozoa

Answer 24

Entamoeba hartmanni, Endolimax nana, Iadomoeba butschlii

Question 25

Which protozoa has a glycogen vauole present

Answer 25

I. butschlii cyst