PROTOZOA Flashcards
Non-motile, Non-Feeding stage, Resistant to acid
Cyst
Motile, Feeding stage, Fragile
Trophozoite
What are the 3 stages of reproduction
Excystation, Encystation, Binary Fission
development/morphologic conversion of trophozoite into cyst
Encystation
mode of reproduction of trophozoite
Binary fission
development/morphologic conversion of cyst into trophozoite
Excystation
General characteristics of protozoa include
- unicellular
- motile
- vary in shape
Parts of cytoplasm
- ectoplasm
- endoplasm
infective stage
Cyst
Most important part of protozoa
Protoplasm
sexual reproduction
syngamy
asexual reproduction
binary fission
example of commonly amoeba
Subphylum darcodina
consider as the only pathogenic intestinal amoeba
Entamoeba histolytica
What is the infective stage or form of Entamoeba histolytica?
quadrinucleated cyst
What are the characteristics of cyst
*spherical shaped
*12-15 um
*4 nuclei (mature)
*Cytoplasm may contain blunt chromatoid bodies
*Phagocytosed erythrocytes (RBC)
What is the mode of transmission for E.H (cyst)
feco-oral
male homosexual
Ingestion
Characteristics of E.H (trophozoite)
size -
nucleus -
cytoplasm -
chromatin -
karyosome -
*pseudopodia with clear ectoplasm
* Size 12-30 micrometers
* Nucleus is hardly visible
*Cytoplasm may contain purple-colored,
ingested red cells
* A single nucleus contains reddish,
concentric karyosome, and peripheral
chromatin evenly distributed
Characteristics of E.H (cyst)
size -
nucleus -
cytoplasm -
chromatin -
karyosome -
- Immature, binucleated cyst in stool specimen (1-4 nucleus)
Trichrome stained - Spherical or ovoidal in shape, 12-20 micrometer in diameter
- Cytoplasm contains reddish-purple colored, cigar-shaped chromatoid bars
- Each nucleus contains reddish, concentric
karyosome, and peripheral chromatin evenly distributed
Used for observation of trophozoite
- saline wet mount
- natural saline solution
- Iodine wet mount