Proteins-Primary Flashcards

1
Q

Proteins expressed at high levels

A

Collagen, hemoglobin

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2
Q

Proteins expressed at low levels

A

Hormones, signaling proteins

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3
Q

Purification and analysis by charge

A

Ion exchange chromatography,
Isoelectric focusing,
Electrophoresis

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4
Q

Protein purification and analysis by Size

A

Dialysis and ultracentrifugation,
Gel electrophoresis,
Gel filtration(size exclusion)
SDS-page

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5
Q

By Charge and Size

A

2D gel electrophoresis

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6
Q

Protein purification and Analysis by Specificity

A

Affinity Chromatography

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7
Q

Protein Purification and Analysis by Polarity

A

Paper chromatography,
Reverse-phase chromatography,
Hydrophobic chromatography

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8
Q

Liquid Chromatography

A

Uses buffer or solvent

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9
Q

Edman uses

A

Enzymes

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10
Q

Mass spec advantages

A
Specific
Sensitive
Identify several peptides from a protein
Multiple proteins at a time in parallel
Type of modification and exact location
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