Proteins And Their Functions Flashcards
Stop codons ?
UAA
UGA
UAG
Start codon?
AUG
Codes for aa methionine
What structure requires the lowest energy
Tertiary
Homomer quaternary structure
Made from same polypeptide chain
Heteromer quaternary structure
Different polypeptide chains
Technologies used to look at protein structure in fine detail
- X-ray crystallography
- Nuclear magnetic resonance
- Cryo-EM
Are proteins rigid structures?
No. Conformation is dynamic.
Induced fit hypothesis
Regulation of proteins (4 steps)
- Synthesis
- Localisation
- Modification
- Degradation
When may synthesis of proteins occur? Examples
- immune response
- differentiation
- in response to signalling
What directs a protein to a desired organelle
Sorting signal
3 methods of protein transport
Nuclear pores
Across membranes
Transport by vesicles - RER to Golgi appa to final organelle
Secretory pathway?
Proteins transported between compartments via transport vesicles
Specialised cells have secretory vesicles which store hormones and enzymes for future use
Types of secretion (2)
o Constitutive secretion:
unregulated
secretion occurs continuously no external signals required
o Regulated secretion:
requires extracellular signal
Difference between secretory and transport vesicles
Secretory contain materials that are to be excreted from the cell
Transport move molecules within the cell
Protein modification?
• Most proteins are post translationally modified in the endoplasmic reticulum (rER)
• Includes disulphide bonds and glycosylation (i.e. sugars added)
• Further modified in the Golgi apparatus