Proteins and Nucleic Acids

Question 1

What mediates Tertiary Structure?

Answer 1

Mediated by side chain interactions- finishes the folding of the polypeptide

Question 2

What type of interaction determines protein folding and tertiary structure?

Answer 2

Hydrophobic interactions

Question 3

If two alpha helices form, how does the structure vary based on where the polypeptide is from?

Answer 3

• If the two alpha helices are from the same polypeptide, we have tertiary structure
• If the two alpha helices are from different polypeptides, we have quarternary structure

Question 4

Primary structure formation _________ _________

While Secondary/Tertiary folding _________ ________

Answer 4

Primary structure formation requires energy

While Secondary/Tertiary folding releases energy

Question 5

What is a coiled coil?

Answer 5

Two alpha helices have hydrophobic amino acids every 4th position

Question 6

What are chaperones?

Answer 6

Specialized proteins that help keep other protein from interacting innapropriately with one another.

Question 7

What is a nucleotide made of?

Answer 7

• 5 carbon sugar,
• Phosphate group
• Nitrogenous base

Question 8

Main structural difference between RNA and DNA (other than number of strands)

Answer 8

RNA has a 2’ hydroxyl that DNA doesn’t have

Question 9

What joins nucleotides together?

Answer 9

Phosphodiester linkages

- 3’OH with 5’ phosphate