Proteins And Enzymes

Question 1

What are the factors that affect enzyme activity?

Answer 1

Enzyme concentration, substrate concentration, temperature, pH

Question 2

How is a control set up in a practical measuring enzyme activity?

Answer 2

Replace the enzyme Solution with distilled water or boiled enzyme solution

Question 3

How can the results of of the practical measuring enzyme activity be used to find the initial rate of reaction?

Answer 3

Plot your results on a graph of ‘rate of reaction’ against ‘ time’. draw a tangent at time=0 to find the initial rate

Question 4

Outline the practical procedure used to measure the effect of temperature on enzyme activity, using trypsin and milk

Answer 4

Immerse equal volumes of trypsin and milk, stored indifferent test tubes, in a water-bath for 5 mins for the temp to equilibrate mix together and immediately start timing, record the time taken for the milk to be completely hydrolysed (colourless) test-at least 5 temperatures with at least 3 repeats

Question 5

How is the rate of reaction calculated from time?

Answer 5

Rate of reaction = 1/ time

Question 6

What is the expect of temp on enzyme activity?

Answer 6

As temp increases,kinetic energy increases so more Es complexes form The rate Reaction up to the optimum temp beyond that, bonds in the enzyme tertiary structures break this changes the shape of the active site. The substrate and enzyme are no longer complementary, so rate of reaction decreases

Question 7

What is the risk associated with handling enzymes?

Answer 7

Students may have allergic reactions to enzymes, so avoid contact with skin and eyes, wear eye protection (lowrisk)