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Clinical Chemistry > Proteins > Flashcards

Proteins Flashcards

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1
Q

Unit For PROTEIN

Unit For Carbs and Lipid

A

g/dL

mg/dL

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2
Q

Proteins Negatively Charged

Positively Charged

A

Alkaline pH

Acidic pH

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3
Q

Also known as Amide Linkages; hold amino acids together

A

Peptide bonds

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4
Q

8 Essential amino acids

A 
Isoleucine
Leucine
Lysine
Threonine
Tryptophan
Phenylalanine
Methionine
Valine
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5
Q

Quaternary structure:

2 subunits ex.

4 subunits ex

A

Creatine kinase

LDH, Hemoglobin

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6
Q

Proteins affected by pH, ionic strength, temp, dielectric constant

A

Differential solubility

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7
Q

Can be accomp thru Ultracentrifugation or dialysis

A

Molecular size

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8
Q

Can be accomp thru Mass Spectro

A

Molecular mass

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9
Q

Done thru Chromatography

A

Surface adsoption

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10
Q

Ion exchange chroma

Electrophoresis

A

Electrical Charge

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11
Q

Protein digestion starts?

Completed?

A

Stomach

Small Int.

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12
Q

Responsible for digesting proteins in stomach

A

Pepsin

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13
Q

6 Major Plasma Protein Fractions

A 
Prealbumin
Albumin
A1 glob
A2 glob
Beta glob
Gamma glob
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14
Q

Gc globulin

A

Alpha 1 globulin

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15
Q

3 alpha 2 globulins

A

Ceruloplasmin
Haptoglobin
A2 Macroglobulin

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16
Q

CRP

Immunoglob are examples of?

A

Gamma globulins

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17
Q

Also known as transthyretin

Faster in migration than Albumin

2nd most predominant protein in CSF

A

PREALBUMIN

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18
Q

Carrier protein for thyroid Hormones and Vitamin A

A

Prealbumin

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19
Q

Normal life span of albumin

A

15-19 days

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20
Q

Decreases during Inflam

Negative Acute Phase reactant

A

Albumin

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21
Q

Dehydration = to ____ serum level of albumin?

A

High albumin

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22
Q 
Edema
Burns
Ascites
Hepatic dse
Level of Albumin?
A

Low Albumin levels

23
Q

Carrier of Steroid hormones

A

A1 acid glycoprotein

24
Q

Decrease AFP Dses

A

Down syndrome

Trisomy 18

25
Q

High AFP levels dses?

A

Germ cell tumors

Hepatocellular carcinoma

26
Q

Account to 90 % beta globulins?

Also known as Siderophilin

A

Transferrin

27
Q

Soluble storage of Fe

A

Ferritin

28
Q

Transports Free Hb

Transports Heme

A

Haptoglobin

Hemopexin

29
Q

An oxidase that decreases in Wilsons Dse

A

Ceruloplasmin

30
Q

Protein present in Plasma

A

Fibrinogen

31
Q

Method used to measure Fibrinogen, uses

Ammonium sulfate
Sodium chloride

A

Parfentjev Method

32
Q

Crp found on what region during electrophoresis??

A

Beta

Some books gamma

33
Q

Bence jones increase in what dse?

A

Multiple Myeloma

34
Q

Protein that dissolves at 100 C

Precipitates at?

A

Bence Jones Protein

40-60 C

35
Q

Heme containing Protein on Striated skeletal muscles and Cardiac

A

Myoglobin

36
Q

Troponin 3 subunits

A

Inhibitory
Tripomyosin binding
Calcium

37
Q

Spx for protein

A

Serum/24 hr Urine/ serous fluids

NO lipemia, Hemolysis

38
Q

Based on digestion of protein, measure nitrogen

A

Kjeldahl method

39
Q

Steps of Kjeldahl method

A

Initial precipitation
Acid digestion
Quantification of Ammonium ions

40
Q

How are ammonium ions quantified in Kjeldhal method??

A

Alkalinization
Distillation
Acid Titration
Nesslerization

41
Q

ALK-Distillation-Titration done using ?

A

Alkali
Boric Acid
HCL

42
Q

Nesslerization done using

A

HgI2

KI

43
Q

Reagents in Buiret Method?

RANK

A

Rochelle Salt
Alkaline CuS04
NaOH
KI

44
Q

Also known as Rochelle salt

Used to complex cupric ion
Prevent Precipitation of cupric ions

A

Sodium potassium tartrate

45
Q

Based in formation of VIOLET colored chelate between Cu ions

A

Biuret Method

46
Q

Spx is mixed with Alkaline Copper Solution ff by Folin Ciocaltue rgnt

A

Lowry Method

47
Q

Used for urine and CSF Protein determinations

A

Salt precipitation Test (Turbudity)

48
Q

Ppts globulins than albumin

A

TCA acid

49
Q

Seen in Px with Liver cirrhosis

Inc IgA in beta and gamma region

A

Beta Gamma bridging effect

50
Q

Seen in cases of monoclonal gammopathy

A

Gamma spike

51
Q

Seen in nephrotic syndrome

A

A2 macroglobulin elevation

Albumin decrease

52
Q

Emphysema

A

A1 antitrypsin deficiency

53
Q

Reference. range

Total protein=

A

6.5- 8.3 g/dL

54
Q

Albumin range

A

3.5 - 5.5 g/dL

Clinical Chemistry (2 decks)

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