Proteins

Question 1

Unit For PROTEIN

Unit For Carbs and Lipid

Answer 1

g/dL

mg/dL

Question 2

Proteins Negatively Charged

Positively Charged

Answer 2

Alkaline pH

Acidic pH

Question 3

Also known as Amide Linkages; hold amino acids together

Answer 3

Peptide bonds

Question 4

8 Essential amino acids

Answer 4

Isoleucine
Leucine
Lysine
Threonine
Tryptophan
Phenylalanine
Methionine
Valine

Question 5

Quaternary structure:

2 subunits ex.

4 subunits ex

Answer 5

Creatine kinase

LDH, Hemoglobin

Question 6

Proteins affected by pH, ionic strength, temp, dielectric constant

Answer 6

Differential solubility

Question 7

Can be accomp thru Ultracentrifugation or dialysis

Answer 7

Molecular size

Question 8

Can be accomp thru Mass Spectro

Answer 8

Molecular mass

Question 9

Done thru Chromatography

Answer 9

Surface adsoption

Question 10

Ion exchange chroma

Electrophoresis

Answer 10

Electrical Charge

Question 11

Protein digestion starts?

Completed?

Answer 11

Stomach

Small Int.

Question 12

Responsible for digesting proteins in stomach

Answer 12

Pepsin

Question 13

6 Major Plasma Protein Fractions

Answer 13

Prealbumin
Albumin
A1 glob
A2 glob
Beta glob
Gamma glob

Question 14

Gc globulin

Answer 14

Alpha 1 globulin

Question 15

3 alpha 2 globulins

Answer 15

Ceruloplasmin
Haptoglobin
A2 Macroglobulin

Question 16

CRP

Immunoglob are examples of?

Answer 16

Gamma globulins

Question 17

Also known as transthyretin

Faster in migration than Albumin

2nd most predominant protein in CSF

Answer 17

PREALBUMIN

Question 18

Carrier protein for thyroid Hormones and Vitamin A

Answer 18

Prealbumin

Question 19

Normal life span of albumin

Answer 19

15-19 days

Question 20

Decreases during Inflam

Negative Acute Phase reactant

Answer 20

Albumin

Question 21

Dehydration = to ____ serum level of albumin?

Answer 21

High albumin

Question 22

Edema
Burns
Ascites
Hepatic dse
Level of Albumin?

Answer 22

Low Albumin levels

Question 23

Carrier of Steroid hormones

Answer 23

A1 acid glycoprotein

Question 24

Decrease AFP Dses

Answer 24

Down syndrome

Trisomy 18

Question 25

High AFP levels dses?

Answer 25

Germ cell tumors

Hepatocellular carcinoma

Question 26

Account to 90 % beta globulins?

Also known as Siderophilin

Answer 26

Transferrin

Question 27

Soluble storage of Fe

Answer 27

Ferritin

Question 28

Transports Free Hb

Transports Heme

Answer 28

Haptoglobin

Hemopexin

Question 29

An oxidase that decreases in Wilsons Dse

Answer 29

Ceruloplasmin

Question 30

Protein present in Plasma

Answer 30

Fibrinogen

Question 31

Method used to measure Fibrinogen, uses

Ammonium sulfate
Sodium chloride

Answer 31

Parfentjev Method

Question 32

Crp found on what region during electrophoresis??

Answer 32

Beta

Some books gamma

Question 33

Bence jones increase in what dse?

Answer 33

Multiple Myeloma

Question 34

Protein that dissolves at 100 C

Precipitates at?

Answer 34

Bence Jones Protein

40-60 C

Question 35

Heme containing Protein on Striated skeletal muscles and Cardiac

Answer 35

Myoglobin

Question 36

Troponin 3 subunits

Answer 36

Inhibitory
Tripomyosin binding
Calcium

Question 37

Spx for protein

Answer 37

Serum/24 hr Urine/ serous fluids

NO lipemia, Hemolysis

Question 38

Based on digestion of protein, measure nitrogen

Answer 38

Kjeldahl method

Question 39

Steps of Kjeldahl method

Answer 39

Initial precipitation
Acid digestion
Quantification of Ammonium ions

Question 40

How are ammonium ions quantified in Kjeldhal method??

Answer 40

Alkalinization
Distillation
Acid Titration
Nesslerization

Question 41

ALK-Distillation-Titration done using ?

Answer 41

Alkali
Boric Acid
HCL

Question 42

Nesslerization done using

Answer 42

HgI2

KI

Question 43

Reagents in Buiret Method?

RANK

Answer 43

Rochelle Salt
Alkaline CuS04
NaOH
KI

Question 44

Also known as Rochelle salt

Used to complex cupric ion
Prevent Precipitation of cupric ions

Answer 44

Sodium potassium tartrate

Question 45

Based in formation of VIOLET colored chelate between Cu ions

Answer 45

Biuret Method

Question 46

Spx is mixed with Alkaline Copper Solution ff by Folin Ciocaltue rgnt

Answer 46

Lowry Method

Question 47

Used for urine and CSF Protein determinations

Answer 47

Salt precipitation Test (Turbudity)

Question 48

Ppts globulins than albumin

Answer 48

TCA acid

Question 49

Seen in Px with Liver cirrhosis

Inc IgA in beta and gamma region

Answer 49

Beta Gamma bridging effect

Question 50

Seen in cases of monoclonal gammopathy

Answer 50

Gamma spike

Question 51

Seen in nephrotic syndrome

Answer 51

A2 macroglobulin elevation

Albumin decrease

Question 52

Emphysema

Answer 52

A1 antitrypsin deficiency

Question 53

Reference. range

Total protein=

Answer 53

6.5- 8.3 g/dL

Question 54

Albumin range

Answer 54

3.5 - 5.5 g/dL