Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Biochemistry LAB > PROTEINS > Flashcards

PROTEINS Flashcards

1
Q

Made up of polymers of amino acids

A

Proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

linkage between amino acids

A

Petide bonds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

_______ depends on the R or the side chain

A

Polarity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

denaturation;

it can only be secondary, tertiary, and quaternary

A

Precipitation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

_____ disrupts hydrogen bond and hydrophobic interactions between R groups

A

HEAT

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Results in heating protein

A

white precipitate and coagulation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

these are positively charged, react with negative part of amino acids (COO-) and neutralizes the protein

A

Heavy metals

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what are the heavy metals involved?

A

Lead acetate
Ferric chloride
Copper sulfate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Alkaloidal

A

Strong mineral salts

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Tannic acid results?

A

white to light brown precipitate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Picric acid results?

A

Yellow flotation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

It splits salt linkage

A

Strong acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Conc. nitric acid results?

A

Cloudy white precipitate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Conc. sulfuric acid results?

A

Brown solution / white precipitate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

denatures protein by disrupting the hydrogen bonds

A

Alcohol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Alcohol results?

A

While solution with white precipitate

17
Q

Detects phenol-derived amino acid (_______)

A

Millons Test

*Tyrosine

18
Q

Composition of Millons Reagent

A

Mercury in Nitric Acid

19
Q

Postive Color for Millons

A

RED

20
Q

Glyoxylic Acid Reaction Principle
The srong acid hydrolyzes the protein
Detects for _______

A

Hopkins Cole Test

*Tryptophan

21
Q

Composition of Hopkins Cole

*Positive color

A

Glyoxlic Acid and Sulfuric Acid

*purple

22
Q

Detection of Glycoproteins

A

Molisch

23
Q

Detection of SH and H Amino Acid

A

Reduced Sulfur

24
Q

Creation of Black Deposits

A

Lead Acetate and Alkaline Solution (NaOH/ KOH)

25
Q

Test with conc. Nitric acid and produces _______ color

A

Xanthoproetic

*yellow

26
Q

Xanthoproetic detects ____

A

Tyrosine and Tryptophan

27
Q

Detection of free amino acids by decarboxylation of a-amino acids

A

Ninhydrin

28
Q

Positive color for Ninhydrin

Except for _______

A

Purple

*Proline no reaxn.

Biochemistry LAB (5 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions