PROTEINS Flashcards
Made up of polymers of amino acids
Proteins
linkage between amino acids
Petide bonds
_______ depends on the R or the side chain
Polarity
denaturation;
it can only be secondary, tertiary, and quaternary
Precipitation
_____ disrupts hydrogen bond and hydrophobic interactions between R groups
HEAT
Results in heating protein
white precipitate and coagulation
these are positively charged, react with negative part of amino acids (COO-) and neutralizes the protein
Heavy metals
what are the heavy metals involved?
Lead acetate
Ferric chloride
Copper sulfate
Alkaloidal
Strong mineral salts
Tannic acid results?
white to light brown precipitate
Picric acid results?
Yellow flotation
It splits salt linkage
Strong acid
Conc. nitric acid results?
Cloudy white precipitate
Conc. sulfuric acid results?
Brown solution / white precipitate
denatures protein by disrupting the hydrogen bonds
Alcohol
Alcohol results?
While solution with white precipitate
Detects phenol-derived amino acid (_______)
Millons Test
*Tyrosine
Composition of Millons Reagent
Mercury in Nitric Acid
Postive Color for Millons
RED
Glyoxylic Acid Reaction Principle
The srong acid hydrolyzes the protein
Detects for _______
Hopkins Cole Test
*Tryptophan
Composition of Hopkins Cole
*Positive color
Glyoxlic Acid and Sulfuric Acid
*purple
Detection of Glycoproteins
Molisch
Detection of SH and H Amino Acid
Reduced Sulfur
Creation of Black Deposits
Lead Acetate and Alkaline Solution (NaOH/ KOH)
