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Biochemistry LAB > Carbohydrates > Flashcards

Carbohydrates Flashcards

1
Q

To hydrolyze the polysaccharide/starch to mono and disaccharide

A

Acid hydrolysis

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2
Q

Reagents in Molisch test

A

Alpha naphthol

H2SO4

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3
Q

Reagent in Molisch test that reacts to furfural

A

Alpha naphthol

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4
Q

Reagent in Molisch test that is an acid that hydrolyses to become furfural

A

H2SO4

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5
Q

Who created Molisch test?

A

Hans Molisch

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6
Q

General test for carbohydrates

A

Molisch test

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7
Q

Molisch test is not for?

A

Trioses or tetroses

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8
Q

Results in molisch test

A

Purple or red in junction

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9
Q

Test where polysaccharides have property of absorption for iodine

A

Iodine test

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10
Q

Color result for Amylose in iodine test

A

Deep blue

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11
Q

Color results for Amylopectin/cellulose in iodine test

A

Red to brown

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12
Q

Color result for Glycogen in Iodine test

A

Red

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13
Q

Reagent in Barfoed’s test

A

Cupric acetate in 1% acetic acid

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14
Q

Cu2O in Barfoed test forms what color of ppt?

A

Red

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15
Q

What is the oxidizing agent for Barfoed’s test?

A

Cu+2

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16
Q

Positive color for Barfoed test, how many minutes?

A

Brick red in:
1-6mins = mono
7-12mins = di

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17
Q

Negative color in Barfoed test

A

Stays blue. No ppt.

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18
Q

Reagents in Benedict’s test

A

Sodium Carbonate
Sodium Citrate
Copper II Sulfate

19
Q

_______ ions are reduced by reducing sugars

A

Cupric ions

20
Q

0.1-0.5 %sugar color

A

Green

21
Q

0.5-1 %sugar color

A

Yellow

22
Q

1-1.5 %sugar color

A

Orange

23
Q

1.5-2 %sugar color

A

Red

24
Q

> 2.0 %sugar color

A

Brick red

25
Q

Negative color for benedict’s test

A

Stays blue

26
Q

Test for reducing sugars

A

Fehling’s test

27
Q

Reagents for fehlings test

A 
Fehlings A (Copper II Sulfate)
Fehlings B (Porassium/ Sodium Tartrate)
w/ NaOH (medium)
28
Q

Positive color in fehling’s test

A

Red

29
Q

Reagents of Bial’s/Orcinol test

A

Orcinol
Hydrochloric acid
Ferric chloride

30
Q

Principle of Bial’s

A

Dehydrate pentose to furfural while hexoses 5 hydroxymethyl furfural

Furfural + orcinol + ferric chloride
=BLUE GREEN

31
Q

5-_________ furfural + ______ and ____________ =?

A

5-Hydroxymethyl furfural + orcinol and ferric chloride = muddy/brownish/red/gray

32
Q

Positive color for Bial’s test

A

Blue/green pentoses

33
Q

Reagents in Tollen’s test

A

AgNO3
NH4OH
KOH/NaOH

34
Q

Test specific only for Aldehyde

A

Tollen’s test

35
Q

Reagents in Seliwanoff’s test

A

0.5% 3N HCl

Resorcinol

36
Q

A Dehydrating reagent in Seliwanoff’s test

A

Hydrochloric acid

37
Q

Condensing reagent in Seliwanoff

A

Resorcinol

38
Q

Principle of Seliwanoff’s test

A

Ketose are rapidly dehydrated than Aldoses

Hydroxymethylfurfural + seliwanoff =reddish ppt

39
Q

Positive tests for Seliwanoff’s test

A 
Ketohexoses = cherry red
Ketopentoses = blue green
Aldohexoses = slower reaction
40
Q

Also known as Osazone test

A

Phenylhydrazone test

41
Q

Test formed with reducing sugars

A

Phenylhydrazone test

42
Q

Maltose shape

A

Flower

43
Q

Lactose shape

A

Mushroom

44
Q

Glucose shape

A

Needle

Biochemistry LAB (5 decks)

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