Carbohydrates

Question 1

To hydrolyze the polysaccharide/starch to mono and disaccharide

Answer 1

Acid hydrolysis

Question 2

Reagents in Molisch test

Answer 2

Alpha naphthol

H2SO4

Question 3

Reagent in Molisch test that reacts to furfural

Answer 3

Alpha naphthol

Question 4

Reagent in Molisch test that is an acid that hydrolyses to become furfural

Answer 4

H2SO4

Question 5

Who created Molisch test?

Answer 5

Hans Molisch

Question 6

General test for carbohydrates

Answer 6

Molisch test

Question 7

Molisch test is not for?

Answer 7

Trioses or tetroses

Question 8

Results in molisch test

Answer 8

Purple or red in junction

Question 9

Test where polysaccharides have property of absorption for iodine

Answer 9

Iodine test

Question 10

Color result for Amylose in iodine test

Answer 10

Deep blue

Question 11

Color results for Amylopectin/cellulose in iodine test

Answer 11

Red to brown

Question 12

Color result for Glycogen in Iodine test

Answer 12

Red

Question 13

Reagent in Barfoed’s test

Answer 13

Cupric acetate in 1% acetic acid

Question 14

Cu2O in Barfoed test forms what color of ppt?

Answer 14

Red

Question 15

What is the oxidizing agent for Barfoed’s test?

Answer 15

Cu+2

Question 16

Positive color for Barfoed test, how many minutes?

Answer 16

Brick red in:
1-6mins = mono
7-12mins = di

Question 17

Negative color in Barfoed test

Answer 17

Stays blue. No ppt.

Question 18

Reagents in Benedict’s test

Answer 18

Sodium Carbonate
Sodium Citrate
Copper II Sulfate

Question 19

_______ ions are reduced by reducing sugars

Answer 19

Cupric ions

Question 20

0.1-0.5 %sugar color

Answer 20

Green

Question 21

0.5-1 %sugar color

Answer 21

Yellow

Question 22

1-1.5 %sugar color

Answer 22

Orange

Question 23

1.5-2 %sugar color

Answer 23

Red

Question 24

> 2.0 %sugar color

Answer 24

Brick red

Question 25

Negative color for benedict’s test

Answer 25

Stays blue

Question 26

Test for reducing sugars

Answer 26

Fehling’s test

Question 27

Reagents for fehlings test

Answer 27

``` Fehlings A (Copper II Sulfate) Fehlings B (Porassium/ Sodium Tartrate) w/ NaOH (medium) ```

Question 28

Positive color in fehling’s test

Answer 28

Red

Question 29

Reagents of Bial’s/Orcinol test

Answer 29

Orcinol Hydrochloric acid Ferric chloride

Question 30

Principle of Bial’s

Answer 30

Dehydrate pentose to furfural while hexoses 5 hydroxymethyl furfural Furfural + orcinol + ferric chloride =BLUE GREEN

Question 31

5-_________ furfural + ______ and ____________ =?

Answer 31

5-Hydroxymethyl furfural + orcinol and ferric chloride = muddy/brownish/red/gray

Question 32

Positive color for Bial’s test

Answer 32

Blue/green pentoses

Question 33

Reagents in Tollen’s test

Answer 33

AgNO3 NH4OH KOH/NaOH

Question 34

Test specific only for Aldehyde

Answer 34

Tollen’s test

Question 35

Reagents in Seliwanoff’s test

Answer 35

0.5% 3N HCl | Resorcinol

Question 36

A Dehydrating reagent in Seliwanoff’s test

Answer 36

Hydrochloric acid

Question 37

Condensing reagent in Seliwanoff

Answer 37

Resorcinol

Question 38

Principle of Seliwanoff’s test

Answer 38

Ketose are rapidly dehydrated than Aldoses Hydroxymethylfurfural + seliwanoff =reddish ppt

Question 39

Positive tests for Seliwanoff’s test

Answer 39

``` Ketohexoses = cherry red Ketopentoses = blue green Aldohexoses = slower reaction ```

Question 40

Also known as Osazone test

Answer 40

Phenylhydrazone test

Question 41

Test formed with reducing sugars

Answer 41

Phenylhydrazone test

Question 42

Maltose shape

Answer 42

Flower

Question 43

Lactose shape

Answer 43

Mushroom

Question 44

Glucose shape

Answer 44

Needle