Proteins

Question 1

What can protein be detected in?

Answer 1

Plasma, Urine, Cerebrospinal fluid, etc.

Question 2

Finish the sentence: the activity of a sample of an ______ is generally related to the amount of protein present in that sample.

Answer 2

Enzyme

Question 3

What is the simplest method of estimating protein concentrations?

Answer 3

Measure the absorbance at 280nm due to the aromatic amino acids (primarily tyrosine and tryptophan), only useful when dealing with pure protein samples

Question 4

The normal value of plasma proteins in humans is 60-80 mg/ml while your protein standards range from 1- 10 mg/ml, what is the solution to this?

Answer 4

Dilute the protein standards 1 in 10

Question 5

How much biuret reagent should be added to the sample and standards and for how long should they be left

Answer 5

Add 3ml of biuret and allow to stand for 20 min

Question 6

When plasm proteins are lower than normal, what is it called and what causes it?

Answer 6

Hypoproteinemia, and is caused by malnutrition, liver disease, and severe burns

Question 7

When plasma proteins are higher than normal, what is it called and what are the causes?

Answer 7

Hyperproteinemia, and is caused by dehydration due to sever vomiting or diarrhoea.