DNA profiling

Question 1

Are the DNA sequences used in DNA profiling coding or non-coding?

Answer 1

Non-coding

Question 2

What are STR’s

Answer 2

Very short DNA sequences that are repeated in direct head-to-tail fashion

Question 3

How are STR alleles detected?

Answer 3

Amplification of copies present in the evidentiary DNA by polymerase chain reaction PCR

Question 4

What is the power of discrimination?

Answer 4

The ability of the typing to discriminate between different individuals

Question 5

Finish this:
The larger the number of ____ typed, the more powerful the ability to discriminate

Answer 5

Loci

Question 6

What should the 5 PCR tubes be labelled?

Answer 6

CS, A, B, C and D

Question 7

How much template DNA should be transferred into each tube?

Answer 7

20 microliters

Question 8

Give me a brief explanation of what to do after adding the template DNA

Answer 8

Transfer 20 microliters of blue MMP (master mix + primer) into each of the 5 PCR tubes, pipette up and down to mix and cap.

Question 9

What does the analysis of the PCR products require?

Answer 9

1X TBE buffer and 3% agarose gel

Question 10

What should be added to lane 1 of the agarose gel?

Answer 10

12 microliters of size marker

Question 11

If the gel should be run with a constant voltage of 10V/cm of agarose gel, what should 15cm of gel be run at?

Answer 11

150V