Proteins Flashcards
What is proteomics
The study of sets of proteins and their properties
What is the proteome ?
The proteome is the entire set of proteins expressed by a genome
Why is the proteome larger than the number of genes ?
Particularly in eukaryotes, because more than one protein can be produced from a single gene as a result of alternative RNA splicing
What are non-coding RNA genes
-Not all genes are expressed as proteins in a particular cell type.
-Genes that do not code for proteins are called non-coding RNA genes
This includes genes that are transcribed to produce;
-tRNA
-rRNA
-RNA molecules that control the expression of other genes.
Why can the set of proteins expressed by a given cell type vary ?
-The set of proteins expressed by a given cell type can vary over time and under different conditions.
Some factors affecting the set of proteins expressed by a given cell type are:
-Metabolic activity of the cell
-Cellular stress
-Response to signalling molecules
-Diseased versus healthy cells
Why do Eukaryotic cells have a system of internal membranes ?
-Because of their size, eukaryotes have a relatively small surface area to volume ratio. The plasma membrane of eukaryotic cells is therefore too small an area to carry out all the vital functions carried out by membranes
-Increases total area of membrane.
What is the endoplasmic reticulum ?
-The endoplasmic reticulum (ER) forms a network of membrane tubules continuous with the nuclear membrane.
-Lipids and proteins are synthesised in the ER
What is the Golgi apparatus ?
The Golgi apparatus is a series of flattened membrane discs.
What are lysosomes ?
Lysosomes are membrane-bound organelles containing a variety of hydrolases that digest proteins, lipids, nucleic acids and carbohydrates.
What do vesicles do ?
Vesicles transport materials between membrane compartments.
Where are lipids synthesised ?
Lipids are synthesised in the smooth endoplasmic reticulum (SER) and inserted into its membrane.
What are the types of endoplasmic reticulum ?
Rough ER (RER) has ribosomes on its cytosolic face while smooth ER (SER) lacks ribosomes.
Where does the synthesis of all proteins begin ?
The synthesis of all proteins begins in cytosolic ribosomes
Where are cytosolic proteins synthesised ?
The synthesis of cytosolic proteins is completed there (the cytosol), and these proteins remain in the cytosol
What are proteins produced by ribosomes bound to the ER generally destined for ?
-Insertion into a membrane
-Secretion from the cell
What are examples of secreted proteins ?
Peptide hormones and digestive enzymes are examples of secreted proteins.
What type of post-translational modification do many secreted proteins require ?
-Proteolytic cleavage is another type of post-translational modification.
-Many secreted proteins are synthesised as inactive precursors and require proteolytic cleavage to produce active proteins.
-Digestive enzymes are one example of secreted proteins that require proteolytic cleavage to become active.
What is the role of the Golgi apparatus ?
As proteins move through the Golgi apparatus they undergo post-translational modification.
Post-translational modification is that a protein’s structure may become modified by adding chemical groups, such as;
-Carbohydrate groups (major modification)
Enzymes catalyse the addition of various sugars in multiple steps to form the carbohydrates
Describe the production of transmembrane proteins
1) Synthesis begins in the cytosolic ribosomes
2) Transmembrane proteins carry a signal sequence, which halts translation and directs the ribosome synthesising the protein to dock with the ER, forming RER
3) A signal sequence is a short stretch of amino acids at one end of the polypeptide that determines the eventual location of a protein in a cell.
4) Translation continues after docking, and the protein is inserted into the membrane of the ER
Describe the movement of proteins (transmembrane) between membranes
1) Once the proteins are in the ER, they are transported by vesicles that bud off from the ER and fuse with the Golgi apparatus.
2) As proteins move through the Golgi apparatus they undergo post-translational modification
3) Molecules move through the Golgi discs in vesicles that bud off from one disc and fuse to the next one in the stack. Enzymes catalyse the addition of various sugars in multiple steps to form the carbohydrates. The addition of carbohydrate groups is the major modification.
4) Vesicles that leave the Golgi apparatus take proteins to the plasma membrane and lysosomes.
5) Vesicles move along microtubules to other membranes and fuse with them within the cell.
Describe the production of secretory proteins.
1) Synthesis begins in the cytosolic ribosomes
2) Presence of a signal sequence, which halts translation and directs the ribosome synthesising the protein to dock with the ER, forming RER
3) A signal sequence is a short stretch of amino acids at one end of the polypeptide that determines the eventual location of a protein in a cell.
4) Translation continues after docking, secreted proteins are translated in ribosomes on the RER and enter its lumen
Describe the secretory pathway
1) Once the secretory proteins are in the RER lumen, they are transported by vesicles that bud off from the ER and fuse with the Golgi apparatus.
2) The proteins move through the Golgi apparatus, undergoing post-translational modification, and are then packaged into secretory vesicles.
3) Molecules move through the Golgi discs in vesicles that bud off from one disc and fuse to the next one in the stack. Enzymes catalyse the addition of various sugars in multiple steps to form the carbohydrates. The addition of carbohydrate groups is the major modification.
4) These vesicles move to and fuse with the plasma membrane, releasing the proteins out of the cell.
What are and what determines the structure of proteins ?
-Proteins are polymers of amino acid monomers
-Amino acid sequence determines protein structure
-Amino acids are linked by peptide bonds (CONH) to form polypeptides
Describe the structure of an amino acid
-Amino acids have the same basic structure, differing only in the R group present.
-R groups of amino acids vary in size, shape, charge, hydrogen bonding capacity and chemical reactivity.
-The wide range of functions carried out by proteins results from the diversity of R groups