Proteins Flashcards

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1
Q

What elements do proteins contain?

A

CHO, nitrogen (+ sometimes sulfur)

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2
Q

What are the monomers of proteins?

A

Amino acids

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3
Q

What do amino acids join together to form?

A

Polypeptides

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4
Q

What is the generalised structure of an amino acid?

A

R
|
H2N—C—COOH
|
H

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5
Q

What bonds are amino acids joined together by?

A

Peptide bonds (-CONH-) formed by condensation reactions

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6
Q

What does the primary structure of a protein represent?

A
  • Refers to the sequence of amino acids in the polypeptide chain
  • Determines specific shape of the protein
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7
Q

What does the secondary structure of a protein represent?

A
  • Represents the folding or coiling of the polypeptide chain (as a result of hydrogen bonding between amino acids)
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8
Q

What does the secondary structure include?

A

Alpha helix OR
Beta-pleated sheet

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9
Q

What does the tertiary structure of a protein represent?

A
  • Formed by further folding and coiling of secondary structure (due to hydrogen bonds, ionic bonds and disulfide bonds)
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10
Q

What is the formation of the bonds in the tertiary structure?

A
  • They form in places determined by the sequence of amino acids in the primary structure
  • They form between the R groups of amino acids
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11
Q

What does the NH2 represent?

A

Amine group

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12
Q

What does the COOH represent?

A

Carboxyl group

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13
Q

What does R represent?

A

A side chain

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14
Q

How do the 20 common amino acids differ?

A

Only by their side group

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15
Q

How are dipeptides formed?

A

By the condensation of two amino acids

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16
Q

How are polypeptides formed?

A

By the condensation of many amino acids

17
Q

What are the characteristics of globular proteins?

A
  • Soluble
  • Biochemical functions
  • Enzymes, myoglobin
18
Q

What is the quaternary structure?

A

When proteins consist of more than one polypeptide chain

19
Q

In the quaternary structure, what bonds are polypeptide chains held together by?

A

Ionic, hydrogen and sometimes disulfide bonds

20
Q

What is denaturation?

A
  • An alteration in the tertiary structure of a protein
21
Q

Is denaturation reversible?

A

No, the loss of the 3D shape is often irreversible and makes it no longer functional

22
Q

What causes denaturation?

A
  • breaking of hydrogen and ionic bonds
  • high temps above optimum
  • extreme changes in pH
  • heavy metals
  • disulfide bonds are not broken at temps that break hydrogen and ionic bonds
23
Q

What is the test for proteins?

A

Biuret test

24
Q

How to carry out the biuret test

A
  • Add biuret reagent
  • purple/lilac = protein present
  • if remains blue = no protein present
25
Q

What are the characteristics of fibrous proteins?

A
  • Insoluble
  • Structural
  • Keratin (hair, collagen)