Proteins Flashcards
Amino acids
A protein monomer
Amino acid structure
NH2 group joined to CH joined to CHR joined to COOH
Dipeptide
2 amino acids joining together by a peptide bond in a condensation reaction (removal of water)
Polypeptide
More than 2 amino acids joined together
Structure of proteins - Primary
The sequence and number of amino acids in a protein
- This happens through many condensation reactions of amino acids (Polymerisation)
- This chain is called a polypeptide
Structure of proteins - Secondary
The shape between the amino acids in the polypeptide chain by the formation of hydrogen bonds
- The shape is either an alpha-helix or a beta-pleated sheet
Structure of proteins - Tertiary
The shape is twisted and folded to create a complex 3D shape
- The structure is maintained by 3 bonds:
- Disulfide bridges = strong and not easily broken
- Ionic bonds = easily broken by changes in pH
- Hydrogen bonds = easily broken
Structure of proteins - Quaternary
The way polypeptide chains are assembled together
- Happens when large proteins form complex molecules containing a number of individual polypeptide chains
Test for proteins
Biuret test
1) Place sample into test tube and add equal volume of sodium hydroxide
2) Add a few drops of dilute copper (II) sulfate solution and mix
3) Purple = positive test (Protein present) Blue = negative test