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CCHM > Proteins > Flashcards

Proteins Flashcards

1
Q

What is the reference value for BUN?

A

8-23mg/dL (2.9-8.2 mmol/L)

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2
Q

It is the major end product of protein and amino acid catabolism.

A

Blood Urea Nitrogen (BUN)

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3
Q

IDMS stands for?

A

Isotope Dilution Mass Spectrometry

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4
Q

Also known as direct method.

A

Chemical method

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5
Q

What is the normal value of creatinine for males?

A

0.9-1.3 mg/dL (80-115 umol/L)

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6
Q

What is the normal value of creatinine for females?

A

0.6-1.1 mg/dL (53-97 umol/L)

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7
Q

End product of muscle metabolism.

A

Creatinine

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8
Q

Give one muscular disease that elevate creatinine levels

A

Muscular dystrophy, poliomyelitis, hyperthyroidism, or trauma

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9
Q

Creatinine is used to monitor this function

A

Renal function

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10
Q

It is the reference method in creatinine determination.

A

IDMS

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11
Q

Kjeldahl Method detects the nitrogen present in the ammonia.

A

TRUE

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12
Q

Lowry Method Principle: Oxidation of phenolic compounds such as tyrosine and tryptophan reduces the PT-PMA reagent (folin-ciocalteu) to give a green color.

A

FALSE (DEEP BLUE COLOR)

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13
Q

Creatinine is not reused in the body’s metabolism, solely as a waste product.

A

TRUE

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14
Q

BUN is freely filtered at the glomerulus but is reabsorbed substantially in the distal convoluted tubule and inner medullary collecting duct.

A

FALSE (Correct answer: PROXIMAL CONVOLUTED TUBULE)

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15
Q

BUN is a good indicator of oxygen intake and the state of hydration.

A

FALSE (Correct answer: NITROGEN)

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16
Q

Turbidimetry is based on the measurement of scattered light by a solution containing fine particles.

A

FALSE (Correct answer: NEPHELOMETRY)

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17
Q

In salt fractionation test, globulins are separated from albumin by a precipitation process using sodium salt.

A

TRUE

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18
Q

Dye-binding method has the highest analytical sensitivity.

A

FALSE (Correct answer: FOLIN-CIOCAL TEU OR LOWRY METHOD)

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19
Q

Ultraviolet Absorption Method Principle: Migration of charge particles in an electrical field.

A

FALSE (Correct answer: SERUM PROTEIN ELECTROPHORESIS)

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20
Q

Kjeldahl Method is the most widely used method, recommended by the International Federation of Clinical Chemistry (IFCC) expert panel.

A

FALSE (Correct answer: BIURET METHOD)

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21
Q

Hepatic disease (BUN)

A

Decreased

22
Q

Pregnancy (BUN)

A

Decreased

23
Q

Chronic renal disease (BUN)

A

Increased

24
Q

Congestive heart failure (Creatinine)

A

Increased

25
Q

Decreased muscle mass (Creatinine)

A

Decreased

26
Q

Condition associated with the decrease in Alpha 1 globulin.

A

ALPHA 1 ANTITRYPSIN DEFICIENCY

27
Q

What are the ions that are bound to albumin?

A

CALCIUM, MAGNESIUM

28
Q

What band does C reactive protein belong to?

A

GAMMA BAND

29
Q

Condition in which there is a beta-gamma bridge formed in the electrophoresis pattern.

A

LIVER CIRRHOSIS

30
Q

Condition where in there is an increase in all of the bands.

A

INFLAMMATION

31
Q

The most common stain used in serum protein electrophoresis?

A

COOMASSIE BRILLIANT BLUE

32
Q

What is the pH of zonal electrophoresis?

A

8.6 pH

33
Q

A stain that can detect small amounts of proteins in a gel and is highly sensitive and selective.

A

AMIDO BLACK

34
Q

It is identified and quantified optically by change in refractive index at the boundaries among protein bands.

A

MOVING BOUNDARY OR FRONTAL ELECTROPHORESIS

35
Q

Used as an anticonvection solid support medium in zonal electrophoresis.

A

FILTER PAPER

36
Q

Two most common protein found in the blood

A

ALBUMIN AND GLOBULIN

37
Q

A condition that is characterized by a buildup of abnormal amyloid deposits in the body. This happen when total protein level increases

A

AMYLOIDOISIS

38
Q

Severely decreased TPAG commonly related that patient has?

A

LIVER CIRRHOSIS

39
Q

It consist of more than 50% of the total serum proteins and also maintains oncotic pressure and transports hormones, vitamins and drugs throughout the body.

A

ALBUMIN

40
Q

Primary purpose of TPAG test?

A

ASSESS/CHECK THE LEVELS OF VARIOUS PROTEINS OF VARIOUS PROTEINS IN THE BLOOD AND IF THE LIVER AND KIDNEY IS FUNCTIONING PROPERTY

41
Q

It is the final product of catabolism of purine and nucleic acids.

A

URIC ACID

42
Q

How many percent or uric acid is being eliminated by renal excretion?

A

70%

43
Q

Kidney disease and/or kidney stones may be caused if uric acid levels are?

A

ELEVATED/INCREASED

44
Q

Reference Method for the analysis of uric acid?

A

ISOTOPE DILUTION MASS SPECTROMETRY

45
Q

What is the required specimen for uric acid? Give one.

A

HEPARINIZED PLASMA, SERUM, OR URINE

46
Q

What is the technique that separates proteins based on their movement in an electric field?

A

SERUM PROTEIN ELECTROPHORESIS

47
Q

What does gel matrix usually contain?

A

AGAROSE OR POLYACRYLAMIDE

48
Q

It provides a medium through which proteins can migrate based on their size and charge, allowing for their separation and analysis.

A

GEL MATRIX

49
Q

In what charge of the electrode does positive charge migrate?

A

NEGATIVE (cathode)

49
Q

The migration of proteins is based on what?

A

SIZE AND SHAPE

CCHM (3 decks)

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