Bilirubin

Question 1

Which chemical is responsible for the yellow color of the urine?

Answer 1

Urobilin

Question 2

It is a bile pigment and is the one responsible for the brown color of the stool.

Answer 2

Stercobilin

Question 3

Which protein transports unconjugated bilirubin to the liver?

Answer 3

Albumin

Question 4

It is the end product of hemoglobin metabolism.

Answer 4

Bilirubin

Question 5

In what form is bilirubin excreted into the bile?

Answer 5

Conjugated

Question 6

What is the normal value of bilirubin for adults?

Answer 6

1.2 mg/dL

Question 7

It is a condition characterized by the yellowing of the skin and eyes due to high bilirubin levels in the body

Answer 7

Jaundice/Hyperbilirubinemia

Question 8

The raw material for bilirubin production.

Answer 8

Heme

Question 9

It is the enzyme responsible for the conversion of B1 to B2.

Answer 9

UDPGT or Uridine Diphosphate Glucoronyl Transferase

Question 10

Which is the first marker of jaundice/hyperbilirubinemia?

Answer 10

Bilirubin in urine (bilirubinuria)

Question 11

State two other names for unconjugated bilirubin.

Answer 11

B1, indirect bilirubin, water insoluble, etc.

Question 12

State two other names for conjugated bilirubin.

Answer 12

B2, direct bilirubin, water soluble, etc.

Question 13

Pre-hepatic hyperbilirubinemia
Cause:
Bilirubin affected:

Answer 13

Cause: too much RBC destructed
Bilirubin affected: B1

Question 14

Hepatic hyperbilirubinemia
Cause:
Bilirubin affected:

Answer 14

Cause: liver disease/defect
Bilirubin affected: B1and B2

Question 15

Post-hepatic hyperbilirubinemia
Cause:
Bilirubin affected:

Answer 15

Cause: physical obstruction
Bilirubin affected: B2

Question 16

What is the end product of direct bilirubin?

Answer 16

Azobilirubin

Question 17

Other term for direct bilirubin?

Answer 17

Conjugated bilirubin

Question 18

What reagent is use for direct bilirubin?

Answer 18

Diazo reagent

Question 19

What is the reactive component of diazo reagent?

Answer 19

Diazonium salt

Question 20

This bilirubin method is measured in the absence of an accelerator.

Answer 20

direct bilirubin method

Question 21

Considered as the most sensitive and most common method used for total bilirubin testing?

Answer 21

Jendrassik-Grof method

Question 22

The main reagent for the Jendrassik Method?

Answer 22

Diazo reagent

Question 23

It buffers the pH of the diazotization reaction?

Answer 23

Sodium acetate

Question 24

Accelerator in Jendrassik-Grof Method?

Answer 24

Caffeine-benzoate

Question 25

Other term for total bilirubin?

Answer 25

Azobilirubin

Question 26

Specimen of choice for bilirubin testing

Answer 26

fasting blood sample, serum

Question 27

A solution that provides alkalinity to the Jendrassik-Grof method?

Answer 27

Alkaline tartrate solution

Question 28

Water insoluble type of bilirubin

Answer 28

unconjugated bilirubin, indirect bilirubin

Question 29

Reference value for total bilirubin

Answer 29

0.2-1.0 mg/dL, 3-17 umol/L

Question 30

After adding caffeine soln or methanol to the serum, how many minutes should we wait before reading the total bilirubin?

Answer 30

15 mins