Proteins Flashcards
How do amino acids/ proteins assist with homeostasis?
they have pH and osmotic affects that assist with homeostasis
structural proteins
form scaffolds of the extracellular matrix and of chromatin
Membrane Proteins
play critical roles in cell signaling
Protein Enzymes
foundation of anabolic and catabolic metabolism
Aberrant Proteins
are at the heart of multiple diseases
enzyme vs protein malfunction
enzyme malfunction = inborn or acquired errors of metabolism
protein malfunction = neurodegenerative conditions and cognitive decline
Name the monomer + examples of:
– proteins
– carbohydrates
– lipids
– nucleic acids
amino acids basic structure
central alpha carbon
carboxylate
amino group
hydrogen
side chain R
Henderson-Hasselbach equation
pH = pKa + log[conjugate base/ conjugate acid]
pI of a protein depends on
the distribution of R groups
– this is because amide bonds between NH3 and COOH groups eliminate their ability to ionize
PI Equation
pI = [pKa (COOH) + pKa (NH3)]/2
– this is for a non-ionizable group R
– about 5.5 for a non-ionizable group R
buffering capacity of amino acids and proteins
+/- 1
protein charge based on pH and PI
pH > pI = protein charge negative
pH < pI = protein charge positive
CO2/ Bicarbonate balance
blood pH is determined by the henderson-hasselbach equation and CO2/ bicarbonate play an important role in buffering capacity
– dissolved CO2 = conjugate acid & dissolved HCO3 = conjugate base
Electrophoresis
done at a pH of 8.6
major plasma proteins separated based on charge differences → migrate towards anode
alterations in normal electrophoretic pattern = stress, trauma, infection, or autoimmune disorder
acidosis and alkylosis
blood levels of CO2 and bicarbonate = critical factors in maintaining normal blood pH (normal = 7.4)
acidosis and alkylosis = serious complications of metabolic or respiratory imbalance → can cause decrease or increase in blood bicarbonate
ionization of drugs affect
it’s oral ability, ability to cross placenta/ blood, and ability to cross the blood brain barrier
drug in a unionized (neutral state) is membrane permeable, but a charged drug is not
neutral form of weak acidic/ basic drug
neutral form of weak acidic drug = conjugate acid
neutral form of weak basic drug = conjugate base
what is critical for protein folding
the side chains R of amino acids that form a protein
hydrophobic vs hydrophilic protein formation
hydrophobic proteins = interior of globular proteins while hydrophilic proteins = exterior because they can form intramolecular hydrogen bonds and have interactions with water molecules
R groups determine what for an enzyme
dictates the specificity of enzyme active sites for the substrate
ex: cysteine = disulfide bonds and proline = break up helix
hierarchy of protein formation
primary structure - amino acid sequence
secondary structure = polypeptide chain interactions (alpha/Beta sheets)
tertiary structure = side chain interactions
quaternary = higher order structures → association of proteins into multimers or fibrils
neurodegenerative diseases occur when
a normally globular protein misfolds = exposes hydrophobic residues that cause aggregation and formation of amyloid fibrils or neurofibrillary tangles
Alzheimers
globular proteins misfolded
AB-42 peptide
Frontotemporal dementias
globular protein misfold
tau protein
transmissible spongiform encephalopathies
globular protein misfold
– prion protein (PrP)
normal functioning PrP = alpha helix; an increase in B-sheet content occurs because of either a mutation in PrP = less stable alpha helix or because of propagation through an infectious B-sheet rich Prp (prion)
reasons for an increase in beta sheets in transmissible spongiform encephalopathies
- mutation in PrP creating a less stable alpha helix
- self propagation through an infectious Beta-sheet rich PrP → known as a prion
stabilization of B-sheets in prion
interlocking hydrogen bonds help stabilization the propagation of B-sheet structure
prions = oligomers
misfolded proteins depending on they type
- amyloid plaques
- neurobirillary tangles
- intraceullalar inclusions (Lewy or pick bodies)
triple helix in collagen distorted by
mutations in glycine →leads to osteogenesis imprefecta
– need glycine for most defining feature of collagen, which is the triple helix
normal sequence: Gly-Pro-Pro/HyP
(severe) anemia genomic cause
a single glutamate to valine mutation at position 6 on the Beta-globin chain renders homoglobin less soluble
(mild) sickle cell anemia genomic causes
glutatmate to lysine mutation at position 6
– lysine chain does not affect aggregation (like Valine does)
cystic fibrosis genomic causse
F508 mutation = mutant of cystic fibrosis transmembrane receptor
CFTR is misfolded and directed towards proteosomes instead of the cell membrane = cyststic fibrosis
– many examples of specific point mutations + examples of heterogenous mutations in protein sequences that give rise to the disease
allostery
small molecule modulators binding to one subunit of a multimer = can trigger a conformational change in other subunits
taut vs relaxed
allosteric proteins and enzymes can adopt a relaxed form = high affinity for substrate
taut form = low affinity for substrate
hemoglobin
a2B2
– four highly helical subunits and 4 heme groups
– binding of one O2 molecule induces a structural change in neighboring subunits that increases their oxygen affinity
– leads to a sigmoidal O2 binding curve
allosteric affectors of hemoglobin
CO2, H+, 2-3-BPG allosteric effectors that stabilize the taut (low) affinity conformer = causes oxygen release
CO binding to the O2 binding site stabilizes the relaxed conformation = prevents oxygen release
heterotropic vs homotropic effectors
heterotropic: bind at a different site from substrate
ex: CO2, H+, and 2,3 BPG bind a different site than O2 in hemoglobin
homotropic: bind at the same site as the substrate
ex: CO binds at the same site as O2 in hemoglobin
model of hemoglobin binding
hemoglobin is allosterically affected = higher affinity with more oxygen binding
relaxed form = when CO2, H+ or 2,3-BPG bind → leads to release of oxygen
sigmoidal O2 curve - hemoglobin
binding of one O2 molecule = structural change in neighboring subunits and increases their oxygen affinity
Bohr equations for hemoglobin
HbO2 + H2 ←→ HbH O2
HbO2 + 2,3-BPG ←→ Hb-2,3-BPG + O2
Hb-NH2 + CO2 ←→ Hb-NH-COO + H+
more acidic = release more O2
more CO2 = release O2 (makes sense as hemoglobin goes through tissue = want to release O2)
hypoxia effects on hemoglobin
- hypoxia will cause increased levels of 2-3-BPG to compensate for oxygen deficiency
hyperventilation effect on hemoglobin
hyperventilation causes alkylosis (body not able to get out enough CO2) this raises the pH of the blood
as a result, this will decrease the release of oxygen in the tissues
Carbon monoxide effect on hemoglobin
O2 starvation due to tight binding of CO at the oxygen binding site and stabilization of the relaxed conformer
Hemoglobinopathies
ex: B-thalassemia and sickle cell disease
these are typically autosomal recessive because one good allele = can make enough protein for normal function
Collagen
most abundant protein in the human body (45 collagen genes)
fibrous protein
types of fibrous proteins
collegen
elastic
fibrillin
alpha-keratin
tropomyosin
Type I - Type IV collagen
– component of
Collagen abnormalities can cause?
aortic and arterial aneurisms
heart valve malfunction
bone fragility
skin distensibility
poor wound healing
joint problems
dislocation of the lens
hallmark of collagen
is a triple helix formed by association of 3 polyproline helices (alpha chains)
polyproline helices of collagen
alpha chains; these give collagen it’s tensile strength
may be homotrimers of same gene product or heterotrimers formed by association of different gene products
(Gly-X-Y)n
(X= Proline, Y= Hydroxyproline)
role of glycine in collagen
glycine only has H as it’ R group = allows the central core of the helix to be tightly wound
– glycine makes up the central core of the helix
Proline and Lysine contribution to collagen
Hydroxylated Proline (Hyp) and Hydroxylated Lysine (Hyl) = hydroxylate version of Pro and Lys
they contribute toward teh triple helix strength through hydrogen bonds on the outside of the polyproline helices
prolyl hydroxylsae
proline → hydroxyproline
cofactors: vitamin C & Fe2+
lysyl hydroxylase
lysine → hydroxylysine
cofactors: vitamin C & Fe2+
lysyl oxidase
collagen microbrils formed by 5 interlaced tripe helices that are stabilized by covalent crosslinks synthesized by lysyl oxidase
cofactors: vitamin B6 & Cu2+
collagen disorders are ___
autosomal dominant
due to dominant negative effect
– mutated alpha chains distort the triple helix and higher order structure even if two of three alpha chains are normal
2 types of autosomal collagen disorders
- dominant negative effect
- null mutation (no collagen produced from that gene)
tropocollagen molecules
arranged in sets of five staggered helices
– supported by covalent crosslinking → known as collagen microfibril
collagen microfibril
5 staggered helices supported by covalent cross linking
collagen fibril
interleaved and interwound collagen microfibrils
collagen fibrils = transversely striated
typically aggregate into collagen fibers = then form bundles and sheaths
which type of collagen is the most vs lease heavily glycosylated
type I = least
type IV = most
vitamin C deficiency
causes scurvy (lysyl hydroxylase and prolyl hydroxylase of collagen synthesis need vitamin C)
Scurvy = accompanied by bleeding gums and poor wound healing