Protein transport

Question 1

clathrin

Answer 1

• Deforms plasma membrane to form vesicle
• Deliver lysosomal enzymes to endosomes from TGN
• Mediate endocytosis

Question 2

Secretory pathway

Answer 2

ER -> Golgi -> vesicle -> plasma membrane

Question 3

Cell pathway

Answer 3

ER -> Golgi -> endosomes -> lysosomes

Question 4

clathrin shape

Answer 4

Triskelion (trimer of clathrin molecules) shape that links together to form polygonal lattices

Question 5

LDL cholesterol pathway

Answer 5

1. ) Receptors gather LDL from the bloodstream
2. ) Clathrin-coated vesicles carry the LDL to the endosome
3. ) Clathrin detaches
4. ) Endosome releases LDL to lysosome
5. ) Empty vesicles return to plasma membrane
6. ) Lysosome breaks down LDL and releases free cholesterol into the cell

Question 6

SNAREs

Answer 6

t-SNAREs on the target membrane attach to v-SNAREs on the vesicle which reduces the energy barrier for the membranes to fuse

Question 7

COPI

Answer 7

Protein coat for vesicles that transfer proteins between Golgi compartments

Question 8

COPII

Answer 8

Protein coat for vesicles that bring new proteins to Golgi from ER

Question 9

Coat formation

Answer 9

initiated by a GTPase

Question 10

Lysosomes

Answer 10

Digest macromolecules with hydrolases

Question 11

Lysosomal enzymes

Answer 11

tagged with mannose-6-phosphate in the Golgi that binds to a receptor in the endosome so it knows to take it to the lysosome and not secrete it