Protein Techniques Flashcards
Insulin has a disulfide bridge connecting the A chain and the B chain. This is a _
covalent post-translational modification
In recombinant proteins only what portion of the insulin gene is cloned?
the active portion
What does Fab stand for?
Fragment has the Antigen Binding site
What does Fc stand for?
Fragment that Crystallizes
A Bcell has antigen receptors on it that recognize
the epitope of an antigen
An epitope is
the specific piece of the antigen that a B cell recognizes via its antigen receptor
Affinity of binding depends on
the tightness of the Fab domain and the epitope
IgG antibodies consist of four chains ___ “blue” and ___ “red” that are linked by
2 heavy, 2 light, linked by disulfide bonds
Polyclonal antibodies
a group of antibodies that each recognize a different antigen
Ascites fluid is ____ and is used to generate ______
an antibody rich fluid found in some tumors, used to generate monoclonal antibodies
Monoclonal antibodies are_____
a single antibody that only recognizes one specific type antigen
Primary antibody= ______
is used to recognize an antigen by binding to it
Secondary antibody=_____
help recognize an antigen by binding to the original antibody that bound the antigen
Proteins can be detected and quantitated by
ELISA
Three types of ELISA
Indirect, Direct, Sandwich
