Protein Synthesis/Mutations/Enzymes Flashcards

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1
Q

which enzyme unwinds DNA and breaks the hydrogen bonds

A

DNA Helicase

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2
Q

Which enzyme adds nucleotides during DNA replication

A

DNA polymerase

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3
Q

When and where does DNA replication occur

A

Interphase; Cytoplasm

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4
Q

Parts of a nucleotide

A

Deoxyribose sugar, a phosphate, and a nitrogen

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5
Q

What pairs to A

A

T

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6
Q

What pairs to C

A

G

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7
Q

What type of chemical bond is between the bases

A

hydrogen bonds

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8
Q

Difference between DNA and RNA

A

DNA has two strands of molecules while RNA only has one. DNA is self-replicating, while RNA can not replicate on its own.

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9
Q

Where is DNA located

A

nucleus

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10
Q

Where is RNA located

A

found in cytoplasm but synthesized in the nucleus

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11
Q

3 types of gene mutations

A

base substitutions, deletions, and insertions

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12
Q

process of transcription

A

transcription uses the information in a gene in the DNA to make a messenger RNA (mRNA) molecule

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13
Q

where does translation take place

A

in the cytoplasm with ribosomes

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14
Q

Process of translation

A

During translation each set of three nucleotides in an mRNA molecule codes for one amino acid in a protein. This process creates codons which creates a polypeptide chain in order to form a protein. mRNA, tRNA, and rRNA are all involved in translation.

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15
Q

What do genes code for

A

proteins

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16
Q

What is a codon

A

A codon is a sequence of 3 DNA or RNA nucleotides that correspond to a specific amino acid

17
Q

What is an anticodon

A

Anticodons are between the nucleotide sequence of the mRNA and the amino acid sequence of the protein

18
Q

How many codons code for 1 amino acid

A

1 codon codes for one amino acid

19
Q

Job of mRNA

A

messenger; encodes amino sequences of a polypeptide

20
Q

Job of tRNA

A

transfers amino acids to the ribosomes during translation

21
Q

Jobs of rRNA

A

ribosomal proteins that make up ribosomes; organelles that translate the mRNA

22
Q

What is an exon? Intron?

A

Exons are segments of mRNA that will be translated “coding region”, while introns are segments of mRNA cut out “non-coding regions.”

23
Q

What is a mutation and when can they occur?

A

Mutations are a permanent change in the DNA sequence. They occur when there are mistakes in the DNA sequence.

24
Q

What type of mutations affect a single gene? multiple genes?

A

Point mutations; frameshift mutations

25
Q

parts of a chemical reaction

A

The reactants; the starting material (molecules/substances) and the products; the final product (outcome/compound)

26
Q

How does a catalyst affect chemical reactions

A

A catalyst affects chemical reactions because it speeds the process of the reaction.

27
Q

What is an enzyme? What is the substrate?

A

Enzymes are the biological substance (proteins) that act as catalysts and help complex reactions occur. Substrates are the biological molecule that the enzyme will work on.

28
Q

What is an active site

A

The active site is a special area that has enzymes grab onto a substrate. The active site changes the shape, holding on to the substrate, prepary to catalyze the reaction.

29
Q

What 3 environmental conditions affect enzyme activity

A

Temperature, pH, and concentration

30
Q

What does it mean when an enzyme is denatured

A

The enzyme is losing its shape and function

31
Q

What is a competitive inhibitor

A

Competitive inhibitor (going in and blocking the active site )

32
Q

What is an allosteric inhibitor

A

The allosteric inhibitor (turning off) binds to an enzyme at a site other than the active site.

33
Q

How do enzymes affect activation energy in a chemical reaction

A

Enzymes are catalysts and catalysts lower the activation energy in reactions. The lower the activation energy the faster the rate will increase. Enzymes speed up the rate of reactions by lowering the activation energy.

34
Q

What happens to chemical bonds in a chemical reaction

A

the chemical bonds that hold the molecules together break apart and form new bonds.