Protein Synthesis/Mutations/Enzymes Flashcards
which enzyme unwinds DNA and breaks the hydrogen bonds
DNA Helicase
Which enzyme adds nucleotides during DNA replication
DNA polymerase
When and where does DNA replication occur
Interphase; Cytoplasm
Parts of a nucleotide
Deoxyribose sugar, a phosphate, and a nitrogen
What pairs to A
T
What pairs to C
G
What type of chemical bond is between the bases
hydrogen bonds
Difference between DNA and RNA
DNA has two strands of molecules while RNA only has one. DNA is self-replicating, while RNA can not replicate on its own.
Where is DNA located
nucleus
Where is RNA located
found in cytoplasm but synthesized in the nucleus
3 types of gene mutations
base substitutions, deletions, and insertions
process of transcription
transcription uses the information in a gene in the DNA to make a messenger RNA (mRNA) molecule
where does translation take place
in the cytoplasm with ribosomes
Process of translation
During translation each set of three nucleotides in an mRNA molecule codes for one amino acid in a protein. This process creates codons which creates a polypeptide chain in order to form a protein. mRNA, tRNA, and rRNA are all involved in translation.
What do genes code for
proteins
What is a codon
A codon is a sequence of 3 DNA or RNA nucleotides that correspond to a specific amino acid
What is an anticodon
Anticodons are between the nucleotide sequence of the mRNA and the amino acid sequence of the protein
How many codons code for 1 amino acid
1 codon codes for one amino acid
Job of mRNA
messenger; encodes amino sequences of a polypeptide
Job of tRNA
transfers amino acids to the ribosomes during translation
Jobs of rRNA
ribosomal proteins that make up ribosomes; organelles that translate the mRNA
What is an exon? Intron?
Exons are segments of mRNA that will be translated “coding region”, while introns are segments of mRNA cut out “non-coding regions.”
What is a mutation and when can they occur?
Mutations are a permanent change in the DNA sequence. They occur when there are mistakes in the DNA sequence.
What type of mutations affect a single gene? multiple genes?
Point mutations; frameshift mutations