protein synthesis and DNA Flashcards

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1
Q

define gene

A

section of DNA on a chromosome coding for one or more polypeptides

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2
Q

define allele

A

one of a number of alternative forms of a gene

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3
Q

define locus

A

position of a gene on a chromosome/DNA molecule

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4
Q

what are the stop codons

A

UAA UAG UGA

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5
Q

what does the stop codon do

A

tells RNA polymerase to detach itself

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6
Q

what is the start codon

A

AUG

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7
Q

what does the start codon do

A

initiates action of RNA polymerase

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8
Q

what is an exon

A

coding sequence that is expressed

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9
Q

what is an intron

A

non coding sequence that’s not expressed

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10
Q

what is splicing

A

when introns are removed from pre mRNA to form mRNA

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11
Q

define genome

A

the complete set of genes or genetic material present in a cell or organism

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12
Q

define proteome

A

the full range of proteins that a cell is able to produce

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13
Q

what does mRNA do

A

brings information from DNA in nucleus to ribosomes in cytoplasm, directs synthesis of polypeptides

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14
Q

what does tRNA do

A

transports amino acids to ribosomes, positions each amino acid at correct place on polypeptide chain

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15
Q

transcription

A
  • hydrogen bonds break (DNA helicase)
  • one DNA strand acts as template
  • free RNA nucleotides align by complementary base pairing
  • uracil pairs with adenine
  • RNA polymerase joins nucleotides by phosphodiester bonds
  • pre mRNA is spliced to form mRNA
  • leaves nucleus via nuclear pore
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16
Q

translation

A
  • mRNA attaches to ribosomes (on rough ER)
  • tRNA anticodons bind to complimentary codons
  • tRNA brings a specific amino acid
  • amino acids join by peptide bonds with the use of ATP (and ribosomal enzyme)
  • tRNA released
  • ribosome moves along the mRNA to form the polypeptide