Protein Structure and Function

Question 1

What are interaction domains?

Answer 1

Independently folded domains of 35-150AAs which can bind their target if produced independently

Question 2

What are the three main methods of protein targeting?

Answer 2

Localisation signal encoded in protein sequence
Post-translational modifications
Binding to scaffold proteins

Question 3

What does the KDEL signal sequence target the protein to?

Answer 3

Retention in the ER

Question 4

What does the KRKR signal sequence target the protein to?

Answer 4

Targeting to the nucleus

Question 5

Where is a hydrophobic peptided targeted towards?

Answer 5

Directs secretion from the cell

Question 6

Which amino acids are proteins predominately phosphorylated on?

Answer 6

Serine and Threonine (Occasionally tyrosine)