Protein Sorting

Question 1

Requirements for protein translocation into organelles

Answer 1

Targetting signal, energy, specific receptor and translocon

Question 2

Ribosomes and pathways for sorting

Answer 2

Co and Post translational

Question 3

What is cotranslational

Answer 3

membrane bound

Question 4

what is post translational

Answer 4

cytosolic

Question 5

Nuclear pore complex components

Answer 5

cytoplasmic filaments, FG nucleoporins, structural nucleoporins, nuclear basket, terminal ring

Question 6

What fo FG nucleoporins doe

Answer 6

line NPC channel and from a hydrophobic gel like matrix that regualtes diffusion and transport

Question 7

How to move proteins for translocation

Answer 7

Use nucleoporins for active transport

Question 8

Nuclear localisation signalling

Answer 8

Short sequence which is recognised

Question 9

Examples of NLS

Answer 9

SV40 T antigen (continuous) and Nucleoplamin (bipartit sequence)

Question 10

Which ones are N-term

Answer 10

ER and mitochondria

Question 11

ER TS

Answer 11

6-12 hydrohpobic amino acids proceeded with 1 or more basic amino acids

Question 12

Mito TS

Answer 12

1 side hydro, 1 side homo, helix 20-50 residues

Question 13

Peroxisome TS

Answer 13

PTS1 signal at C-term, PTS2 at N-term

Question 14

Nucleus TS

Answer 14

multiples

Question 15

Importin pathway energy

Answer 15

GTP in particular Ran GTP

Question 16

How does Importin pathway work

Answer 16

GTP causes conformational change, alpha binds NLS and B interacts with FG

Question 17

What is the importin parthway

Answer 17

nuclear import pathway

Question 18

Where are mitochondrial proteins synthesised

Answer 18

cytoplasm

Question 19

Targetting sequence for mitochondria

Answer 19

MTS

Question 20

First place proteins travel for mitochondria

Answer 20

First travel into intermembrane space of mitochondria

Question 21

What chaperones do Mitochondrial proteins have and why

Answer 21

HSP70 which maintain unfolded state

Question 22

What recognises MTS

Answer 22

TOM 20 and 22 tom stands for outer membrane

Question 23

What crosses helps move protein when in mitochondiral membrane

Answer 23

Tim 23/17 and Tim 44

Question 24

When in mitochondrial matrix what happens

Answer 24

HSP70 molecules pull it through and then it is cleaved