Protein Sequencing (Dr.Miles TAMU)

Question 1

Primary Structure

Answer 1

is the linear sequence of amino acids

Question 2

Secondary Structure

Answer 2

the spatial conformation of the polypeptide’s backbone without regard to the conformation of the side chains. Includes α−Helix, β−Sheets and β−Turns.

Question 3

Tertiary Structure

Answer 3

refers to the three dimensional structure of the entire polypeptide including the side chains

Question 4

Quaternary Structure

Answer 4

many proteins are composed of 2 or subunits. The quaternary structure is
the spatial arrangement of these subunits

Question 5

What was the first peptide to have it’s primary structure determined

Answer 5

Insulin

Question 6

Trypsin

Answer 6

Digestive enzyme that will cleave at the C-terminal side of lysine(k) and Arginie(R) unless followed by a proline

Question 7

Elastase

Answer 7

cleaves Gly(G), Ala(A), Serine(S), and Val(V) unless followed by proline

Question 8

Chymotrypsin

Answer 8

Cleaves Phe(F), Trp(W), and Tyrosine(Y) unless followed by proline

Question 9

Cyanogen Bromide

Answer 9

Will cleave Methionine (M)