Protein sequencing

Question 1

What is the reagent used to reduce the disulfide bonds in a protein sequence?

Answer 1

BME

Question 2

What is used to prevent the reformation of the disulfide bond?

Answer 2

iodoacetic acid

Question 3

What two amino acids does the enzyme trypsin cut after?

Answer 3

Arg and Lys

Question 4

What does an enzyme bind to that is agonist?

Answer 4

It binds to substrate

Question 5

What does an enzyme bind to that is antagonist?

Answer 5

It binds to inhibitors.

Question 6

What is the equation for Kd

Answer 6

Kd = [P][L]/[PL]

Question 7

what does the Kd provide?

Answer 7

its the dissociation constant that provides the equilibrium constant between a ligand binding to protein

Question 8

What are the four key points regarding Kd?

Answer 8

1. It’s a concentration is moles/L
2. Gives conc. of ligand that saturates 50% of the binding sites
3. Almost all binding sites are full at ten times the Kd
4. Kd is related to the Gibbs free energy by G= -RT*ln(Kd)