Cards for first midterm (summer)

Question 1

The name given to an ordered water structure around a hydrophobic molecule

Answer 1

Clathrate cage

Question 2

List, in order, the noncovalent bonds by strength

Answer 2

1. ionic interaction
2. Hydrogen bond
3. Dipole - dipole interaction
4. London dispersion

Question 3

What is the Kw value?

Answer 3

1.0 E - 14

Question 4

What is the acid dissociation const.

Answer 4

Ka = (H+)(A-)/ (HA)

Question 5

Derrive Henderson Hasselbalch

Answer 5

Okay

Question 6

At what pH does alkalosis and acidosis begin

Answer 6

7.6, 7.1

Question 7

What is the more stable form of the nuceic acids

Answer 7

Keto form, not enol form

Question 8

the two forces that hold a helix together?

Answer 8

Hydrogen and van der waals

Question 9

What is the Tm

Answer 9

The point at which half of DNA is denatured

Question 10

What is the diameter of B-DNA?

Answer 10

20A

Question 11

What is the base pairs per turn of B-DNA?

Answer 11

10

Question 12

What is the helical twist of a base pair of B-DNA?

Answer 12

36 degrees

Question 13

What is the pitch of B-DNA?

Answer 13

34A

Question 14

What is the van der waals thickness of an aromatic base?

Answer 14

3.4 A

Question 15

What are the two types of select-able markers for plasmid selection?

Answer 15

chromogenic and antibiotic

Question 16

What are the four ingredients for PCR

Answer 16

DNA strand, primer, dNTPs, and Taq

Question 17

The three steps of sequencing

Answer 17

1. cleave polymer
2. Determine sequence of each fragment
3. Determine overall nucleotide sequence

Question 18

What is the charge of DNA in the electrophoresis gel?

Answer 18

negative

Question 19

what is the average length of a plasmid

Answer 19

100kb

Question 20

What is ddNTP

Answer 20

nuceotides lacking 3” alcohol

Question 21

What is a reed?

Answer 21

partial DNA sequence