Protein Purification Methods

Question 1

What amino acid side chains are susceptible to Trypsin?

Answer 1

Arg (R)
Lys (K)

SAME AS METHYLATION

Question 2

What amino acid side chains are susceptible to Chymotrypsin?

Answer 2

Phe (F)
Trp (W)
Tyr (Y)

SAME AS UV

Question 3

What amino acid side chain is susceptible to Clostripain?

Answer 3

Arg (R)

PAIN

Question 4

What amino acid side chains are susceptible to Staph.?

Answer 4

Asparaginw (N)
Glu (Q)

As
G

Question 5

What amino acid is susceptible to Cyanogen Bromide?

Answer 5

Met

Question 6

What amino acid side chain is susceptible to Ubiquitination?

Answer 6

Lysine (K)

Question 7

What amino acid side chain is susceptible to N-linked glycosylation?

Answer 7

AsparagiNe (N)

Question 8

What amino acid side chains are susceptible to O-linked glycosylation?

Answer 8

Ser (S)
Threonine (T)

ST

Question 9

What amino acid side chain is susceptible to Myristolation?

Answer 9

Glycine (G)

Question 10

What amino acid side chains are susceptible to phosphorylation?

Answer 10

Ser (S)
Threonine (T)
Tyrosine (Y)

Question 11

What amino acid side chain is susceptible to Prenylation?

Answer 11

Cys (C)

Question 12

What amino acid side chain is susceptible to Palmitoyl?

Answer 12

Cys (C)

Question 13

What amino acid side chains are susceptible to acetylation?

Answer 13

Lys (K)
N terminal of peptide

Question 14

What amino acid side chains are susceptible to methylation?

Answer 14

Arg (R)
Lys (K)

SAME AS TRYPSIN