Protein purification and characterisation Flashcards
True or false: Key metabolic processes are common to many organisms
True. It is seen from the biochemistry (proteins)
Proteins are how many percent of all cellular systems?
60-70%
Proteins consist of linear polymers called…
polypeptides
Proteins are … and never branched
Linear
What are the bonds called that link amino acids together?
Peptide bonds
What are the individual amino acids within a protein called?
Residues
What is the smallest known protein of only nine residues called?
Oxytoxin
The largest protein titin is over 25000 residues and is found in the…
muscles
How many residues do proteins generally have?
100 to 1000
In the absence of stabilising forces how many residues are needed to adopt stable 3D structure in water?
40
Edman degradation is done by systematically removing the amino acids one at a time, why is it done?
To determine protein sequence
What are the four levels of protein structure called?
Primary, secondary (alpha helix, beta sheets), tertiary (domains), quaternary (two or more forming complexes)
Experiments have shown that the final 3D tertiary structure is determined by the
primary structure, the amino acid sequence
The shape of the protein determines its…
function
T/F: Proteins play a central role in all biological processes
T
What is the name of the type of protein that catalyses
Enzyme
What is the name/example of the type of protein that transports and stores
Haemoglobin, myoglobin
What is the name of the type of protein that acts as mechaninal support
Collagen
What is the name of the type of protein that acts as immune protection
Immunoglobin
What is the name of the type of protein that transmits nerve pulses
Rhodopsin
What is the name of the type of protein that controls growt and differentiation
Growth factor
What is an example of a protein function?
Regulate and repair DNA
Structure of a protein defines
interactions
site specificity
hydrodynamics
association
A protein has non-covalent bonds too, what are these called?
Ionic bonds
Hydrogen bonds
Van der Waals forces