Protein in nanomaterials 2.0 Flashcards
Advantages of the use of protein materials for drug delivery
- Biocompatible
- Biodegradable
- Useful intrinsic properties
- High drug binding capacity
- Renewable sources
Factors that are important in modifying and application of protein materials
- Location
- Functionality
- Stability
Synthesis methods for the whole protein-based nanomaterials
- Desolvation
- Coacervation
- Nanospray
- Nano-precipitation
- Emulsification
- Self assembly
Virus particles as nanomaterials
Virus like particles (VPL) are made out of capsid proteins and contain no viral RNA. The architecture and it not containing viral RNA makes it perfect for targeted immunization without real danger
Morphology and structure of VPL in relation to nanomaterials
VPLs are often very symmetrical with repetitive structures while regular nanomaterial lack that
Modifications in proteins for stucturing in nanomaterials
- Incorporating Non canonical amino acids
- Crispr CAS9
Elastin-like peptides, Their biological function, (bio) synthesis and selforganization
Elastin is a major component of elastic fibers found in connective tissue like the skin, blood vessels and lungs.
In the body elastin in produced by fibroblasts. ELPs are produces by recombinant DNA technology. This is done by using bacteria or yeast.
Self assembly: Below the transition temperature ( Tt). EPLs remain soluble in solution. When the temperature exceeds the Tt, ELPs undergo phase transistion forming a gel-like stucture
Applicability ELP materials in drug delivery
ELP designed to encapsulate drug at lower temprature. When target site with higer temperature the ELPs release the drug.
Protein materials as functional handles in tageted drug delivery
Antibodies can be used for targeted drug delivery. Targeting peptides like RGD van aslo be used.
Techniques to determine the activity of proteins
ELISA: Find the binding of a target protein/peptide to certain proteins.
Phage display: Connection between genotype and fenotype
Sequence alignment: To check importance of a protein through differt species
Assembly methods of ELP and VLP nanomaterials
ELPs are assemble Through selfassembly.
VPLs are assembled through expression of viral stuctural proteins in host cells ( bacteria/yeast). This leads to the spontaneous assemlby of VPLS
