Protein Function Flashcards
A molecule that binds to a protein is called
Ligand
A region in the protein where the ligand binds is called
binding site
Ligands bind to the same noncovalent/ different covalent forces that dictate protein structure?
same noncovalent
characteristics of ligand binding
reversible
specific
transient
What expresses how quickly a reaction takes place or how frequently?
dissociations
What % tells us how good a protewin is bound
50%
since [P] is not always known, what is a convenient way to express theta in terms of
[L}
why is the [P] not always known
biochemist only care about amount of active protein
[L] is a concentration, measured in
M (molar)
Unit of Kd
M
The smaller the Kd, the – affinity
higher the affinity, the tighter the binding
Is binding better always good for the cell
no, sometimes u need weak higher Kd
Oxygen-binding proteins are called
Globins
Because we want to avoid free radicals in solution, what organometallic compounds do we use?
Fe2+ in heme oxidized to Fe3+
The main oxygen storage protein is called
Myoglobin
The circulating oxygen-binding protein is called
hemoglobin
Hemoglobin can change between which state
Low affinity where it gives up oxygen
High affinity where it binds oxygen
Where is myoglobin found
mammalian muscle
Metalloprotein Fe2+ is contained in a prostethic group called
Heme
Waht is the structure of protoporphyrins
tetrapyrroles
What does X group determine
the type of protoporphyrin we are talking about
How many nitrogen provide coordination sites to heme iron
4
In free heme, what happens to the 2 other coordination sites
they are available for binding
What other molecules do heme bind to
CO which binds stronger, killing people
