Protein Folding Flashcards
1
Q
What is folding restricted by?
A
- Rigidity of peptide
- Chemical & physical properties of side chains
2
Q
What proteins support effective folding?
A
Molecular chaperones and Chaperonins
3
Q
Amyloid disease definition
A
Fragments of normal proteins produce amyloid fibrils
4
Q
Alzheimer’s Biochemical Basis
A
- Amyloid disease
- Fragment from normal membrane protein (Amyloid Precursor Protein APP) accumulates and aggregates
- Forms insoluble fibrils of amyloid beta protein in brain
- Fibrils aggregate to form plaques
- Plaques damage and destroy neurones
5
Q
Prion diseases definition
A
Infectious protein agent causes conformational change in normal protein
6
Q
Creutzfeldt Jacob Disease Biochemical Basis
A
Involves interaction between PrP^Sc (form of prion protein that causes disease) and normal membrane protein PrP^c
- PrP^Sc has identical 1ary structure but higher proportion of beta-pleated sheet
- When solute PrP^c comes into contact with PrP^Sc it causes PrP^c to acquire abnormal PrP^Sc structure
- Forms soluble aggregates of protein
7
Q
How do molecular chaperones assist folding?
A
Bind to short segments of protein to facilitate correct folding
8
Q
How do chaperonins assist folding?
A
Form folding chambers which provide stable environment to encourage correct folding
