Protein Expression + Engineering Flashcards
What is the promoter
What is an operator
What is the RBS
What is the ATG site
What is the antibiotic resistance gene for needed for in recombinant plasmids plasmids
What are the different genetic elements involved in expression of a plasmid encoded protein
How does the lac operon work
What are the 4 phases of bacterial growth
Lag phase
Exponential phase
Stationary phase
Decline/ death phase
What is the lag phase
What is the exponential phase
What is the stationary phase of bacterial growth
When is the optimal time for expressing a protein of interest
What are the limitations to recombinant protein expression + solution
What is auto induction
Why can expression of recombinant proteins be toxic to transformed bacteria
How can the problem of expression of recombinant proteins being toxic to the transformed bacteria be solved
Give some examples of E. coli strains and their key features
What is the first step of cloning
Why is taq pol useful
How does DNA topo 1work
How does traditional cloning work
What does ligand independent cloning do
What is the process of ligand independent cloning
How does gateway technology cloning work
What is a fusion protein
What are commonly used tags in cloning
Explain this E. coli expression vector
How are forward primers designed
How are reverse primers designed