Protein Based Techniques

Question 1

Protein based analyses are

Answer 1

SDS-PAGE
2D Protein Electrophoresis
…

Question 2

SDS-PAGE

Answer 2

Separates proteins based on size
SDS is an ionic detergent

Question 3

2D Protein Electrophoresis

Answer 3

Separates proteins firstly on the basis of isoelectric points (pI), then size

Question 4

Dimensions of 2D Protein Electrophoresis

Answer 4

1D: separation of proteins based on pI points (IEG or IPG)
2D: separation of proteins based on sizes (SDS-PAGE)

Question 5

Important points of 2D Protein Electrophoresis

Answer 5

Best way to separate protein molecules
Exact identification of proteins with same size and different aa sequences

Question 6

Genomics, Transcriptomics, Proteomics

Answer 6

Genomics: targets DNA
Transcriptomics: targets expressed genes
Proteomics: targets proteins

Question 7

Genes (expressed or suppressed) in proteins?

Answer 7

All of mRNA is converted to proteins
All of them are EXPRESSED genes