Hybridization Flashcards
1
Q
What is hybridization
A
By using the probe, you can find the target. Whenever probe finds the target, they bind to it
2
Q
How can you prepare your own probe
A
If you know the sequence of the target
(Ex. If you have the specific probe for E. coli, you can find it anywhere)
3
Q
2 types of hybridization
A
Strong hyb
Weak hyb (gives positive false results)
4
Q
How to get rid of weak hybridization
A
Sample should be washed to remove weak hyb
5
Q
Hybridization methods
A
Southern (DNA) & Northern (RNA) blot hybrid
Western blot hyb (proteins)
Dot and Slot blot hyb
Colony blot hyb —> only this is culture-dependent
6
Q
What is blotting!!!!!!!!!!
A
Transferring the bands from the gel to nitrocellulose membranes to run hybridization with probes
